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Acid and Pepsin Soluble Collagens from Skin By-product of Red-bellied Pacu (Piaractus brachypomus): Extraction and Comparative Characterizations Towards Finding Substitute to Bovine and Porcine Collagen.

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Relation http://ir.cftri.com/14804/
https://doi.org/10.1080/10498850.2021.1884374
 
Title Acid and Pepsin Soluble Collagens from Skin
By-product of Red-bellied Pacu (Piaractus
brachypomus): Extraction and Comparative
Characterizations Towards Finding Substitute to
Bovine and Porcine Collagen.
 
Creator Lidiya, C. Johny
Tanaji, G. Kudre
Suresh, P. V.
 
Subject 03 Proteins
Fish
 
Description This investigation focused on the sustainable valorization of red-bellied pacu
(RBP) skin towards finding an alternative to bovine and porcine collagen.
Acid-soluble collagen (ASC, 45.8 ± 0.7%) and pepsin soluble collagen (PSC,
57.8 ± 1.9%) were successfully extracted from RBP skin and comparatively
elucidated their characteristics and in-vitro fibril-forming potential. The
extracted collagens were characterized as type I collagen and confirmed
the well-preserved triple-helical configuration by sodium dodecyl sulphate
polyacrylamide gel electrophoresis and Fourier transform-infrared spectro�scopy analysis. Both ASC and PSC exhibited relatively better thermo-stability,
and the denaturation temperatures were found to be 35.5–35.29 °C by
differential scanning calorimetry analysis. Both collagens displayed higher
(p ≤ 0.05) solubility at low NaCl concentrations. The extracted ASC and PSC
showed the same degree of fibril-forming capacities and confirmed well�defined fibril morphologies. Overall, the results showed the possibilities of
effectively exploring RBP skin as a potential alternative source for realistic
and safe collagen for food, nutrition, pharmaceutical, and other applications.
 
Date 2021
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/14804/1/JOURNAL%20OF%20AQUATIC%20FOOD%20PRODUCT%20TECHNOLOGY%202021.pdf
Lidiya, C. Johny and Tanaji, G. Kudre and Suresh, P. V. (2021) Acid and Pepsin Soluble Collagens from Skin By-product of Red-bellied Pacu (Piaractus brachypomus): Extraction and Comparative Characterizations Towards Finding Substitute to Bovine and Porcine Collagen. Journal of Aquatic Food Product Technology. ISSN ISSN: 1049-8850