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Methanolic extract of Phlogacanthus thyrsiflorus Nees leaf induces apoptosis in cancer cells

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Title Methanolic extract of Phlogacanthus thyrsiflorus Nees leaf induces apoptosis in cancer cells
 
Creator Chanu, Khangembam Victoria
Devi, Leishangthem Geeta
Srivastava, Sandeep Kumar
Kataria, Meena
Thakuria, Dimpal
Kumar, Satish
 
Subject Annexin V/PI staining
Anticancer
Caspase-3
Cytotoxicity
DNA fragmentation
Electron microscopy
Nuclear fragmentation
Tumor
 
Description 153-161
Phlogacanthus thyrsiflorus Nees is a medicinal herb commonly used in traditional folk medicine, and it is known to possess anticancer activity. Here, we explored the anticancer properties of methanolic extract of P. thyrsiflorus leaves in HeLa and MCF-7 cell lines. We observed nuclear fragmentation as indication of apoptosis in the MPT treated cancer cells using haematoxylin and eosin (H&E) as well as fluorescent dye. DNA from the treated cells showed characteristic laddering of DNA fragments in agarose gel electrophoresis. Cell populations undergoing secondary necrosis following apoptosis could also be detected in FACS by annexin V/propidium iodide (PI) staining. Activated caspase-3 in the treated HeLa cells was detected by polyclonal anti-caspase-3 antibody utilizing immunocytochemistry. Using transmission electron microscopy, sub-cellular changes like rough endoplasmic reticulum, swollen mitochondria, distorted mitochondrial membrane, loss of cristae and matrix were observed in the treated HeLa cells. Extensive plasma membrane blebbing was also observed by scanning electron microscopy. Our findings support that Phlogacanthus thyrsiflorus leaves are natural source of potent anticancer agent.
 
Date 2021-03-09T05:05:24Z
2021-03-09T05:05:24Z
2021-03
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscair.res.in/handle/123456789/56400
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJEB Vol.59(03) [March 2021]