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The quest for reusability: The facile and stable immobilization of papain on cysteine functionalized iron oxide nanoparticles activated glass surface

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Title The quest for reusability: The facile and stable immobilization of papain on cysteine functionalized iron oxide nanoparticles activated glass surface
 
Creator Chauhan, Shraddha
Upadhyay, Lata Sheo Bachan
 
Subject Cysteine
Iron oxide nanoparticle
Papain immobilization
 
Description 141-147
The study displays the immobilization of papain on cysteine functionalized iron oxide nanoparticle coated glass beads. Glass beads were treated with (3-mercaptopropyl) trimethoxysilane and iron oxide nanoparticles capped with cysteine, to create a layer of cysteine on the surface of glass bead. This functionalized glass bead surface was further used to immobilize papain through glutaraldehyde treatment. The average size of cysteine capped iron oxide nanoparticles were found to be 50 nm. The binding of cysteine through the iron oxide nanoparticles was validated by Fourier transform infra-red spectroscopy. The activity of enzyme was found to be stable at variable temperature and pH conditions. The covalently immobilized enzyme on the glass bead sustained high enzyme activity and could be reused for 5 times without losing its activity. The immobilized papain retained 81% of its initial activity after 5 consecutive cycle. The storage stability analysis of the immobilized system revealed that it is stable for 6 months without loss in its activity. An average of 1.8 mg papain was successfully immobilized per gram of glass beads. In this case cysteine emerges as a new and effective medium for immobilizing biomolecules as it provides high efficiency towards immobilization.
 
Date 2021-03-11T07:17:09Z
2021-03-11T07:17:09Z
2021-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscair.res.in/handle/123456789/56486
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.58(2) [April 2021]