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The quest for reusability: The facile and stable immobilization of papain on cysteine functionalized iron oxide nanoparticles activated glass surface

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Title Statement The quest for reusability: The facile and stable immobilization of papain on cysteine functionalized iron oxide nanoparticles activated glass surface
 
Added Entry - Uncontrolled Name Chauhan, Shraddha
Upadhyay, Lata SheoBachan
 
Uncontrolled Index Term Cysteine; Iron oxide nanoparticle; Papain immobilization
 
Summary, etc. The study displays the immobilization of papain on cysteine functionalized iron oxide nanoparticle coated glass beads. Glass beads were treated with (3-mercaptopropyl) trimethoxysilane and iron oxide nanoparticles capped with cysteine, to create a layer of cysteine on the surface of glass bead. This functionalized glass bead surface was further used to immobilize papain through glutaraldehyde treatment. The average size of cysteine capped iron oxide nanoparticles were found to be 50 nm. The binding of cysteine through the iron oxide nanoparticles was validated by Fourier transform infra-red spectroscopy. The activity of enzyme was found to be stable at variable temperature and pH conditions. The covalently immobilized enzyme on the glass bead sustained high enzyme activity and could be reused for 5 times without losing its activity. The immobilized papain retained 81% of its initial activity after 5 consecutive cycle. The storage stability analysis of the immobilized system revealed that it is stable for 6 months without loss in its activity. An average of 1.8 mg papain was successfully immobilized per gram of glass beads. In this case cysteine emerges as a new and effective medium for immobilizing biomolecules as it provides high efficiency towards immobilization.
 
Publication, Distribution, Etc. Indian Journal of Biochemistry and Biophysics (IJBB)
2021-03-11 00:00:00
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/IJBB/article/view/35100
 
Data Source Entry Indian Journal of Biochemistry and Biophysics (IJBB); IJBB Vol. 58 (2) [April 2021]
 
Language Note en