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Transdermal Immunization of Elastic Liposome-Laden Recombinant Chimeric Fusion Protein of P. falciparum (PfMSP-Fu(24)) Mounts Protective Immune Response

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Transdermal Immunization of Elastic Liposome-Laden Recombinant Chimeric Fusion Protein of P. falciparum (PfMSP-Fu(24)) Mounts Protective Immune Response
 
Creator Chaudhari, Ramesh
Tandel, Nikunj
Sahu, Kiran
Negi, Sushmita
Bashir, Hilal
Rupareliya, Arzu
Mishra, Ravi Pn
Dalai, Sarat K.
Tyagi, Rajeev K
 
Subject QR Microbiology
 
Description Transdermal immunization exhibits poor immunogenic responses due to poor permeability of antigens through the skin. Elastic liposomes, the ultradeformable nanoscale lipid vesicles, overcome the permeability issues and prove a versatile nanocarrier for transcutaneous delivery of protein, peptide, and nucleic acid antigens. Elastic liposome-mediated subcutaneous delivery of chimeric fusion protein (PfMSP-Fu(24)) of Plasmodium falciparum exhibited improved immunogenic responses. Elastic liposomes-mediated immunization of PfMSP-Fu(24) conferred immunity to the asexual blood-stage infection. Present study is an attempt to compare the protective immune response mounted by the PfMSP-Fu(24) upon administered through transdermal and intramuscular routes. Humoral and cell-mediated immune (CMI) response elicited by topical and intramuscularly administered PfMSP-Fu(24)-laden elastic liposomes (EL-PfMSP-Fu(24)) were compared and normalized with the vehicle control. Sizeable immune responses were seen with the transcutaneously immunized EL-PfMSP-Fu(24) and compared with those elicited with intramuscularly administered antigen. Our results show significant IgG isotype subclass (IgG1and IgG3) response of specific antibody levels as well as cell-mediated immunity (CMI) activating factor (IFN-gamma), a crucial player in conferring resistance to blood-stage malaria in mice receiving EL-PfMSP-Fu(24) through transdermal route as compared to the intramuscularly administered formulation. Heightened immune response obtained by the vaccination of EL-PfMSP-Fu(24) was complemented by the quantification of the transcript (mRNA) levels cell-mediated (IFN-gamma, IL-4), and regulatory immune response (IL-10) in the lymph nodes and spleen. Collectively, elastic liposomes prove their immune-adjuvant property as they evoke sizeable and perdurable immune response against PfMSP-Fu(24) and justify its potential for the improved vaccine delivery to inducing both humoral and CM immune response.
 
Publisher MDPI
 
Date 2021-02-05
 
Type Article
PeerReviewed
 
Relation https://www.mdpi.com/2079-4991/11/2/406
http://crdd.osdd.net/open/2659/
 
Identifier Chaudhari, Ramesh and Tandel, Nikunj and Sahu, Kiran and Negi, Sushmita and Bashir, Hilal and Rupareliya, Arzu and Mishra, Ravi Pn and Dalai, Sarat K. and Tyagi, Rajeev K (2021) Transdermal Immunization of Elastic Liposome-Laden Recombinant Chimeric Fusion Protein of P. falciparum (PfMSP-Fu(24)) Mounts Protective Immune Response. Nanomaterials, 11 (2). pp. 1-23.