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Genotyping by Sequencing SNP data for Nitrogen Use Efficiency in diverse Sorghum genotypes

ICRISAT Dataverse Dataverse OAI Archive

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Title Genotyping by Sequencing SNP data for Nitrogen Use Efficiency in diverse Sorghum genotypes
 
Identifier https://doi.org/10.21421/D2/BYZY2F
 
Creator Gupta Rajeev
Deshpande Santosh
Srikanth Bollam
Laavanya Rayaprolu
Abhishek Rathore
Roma Rani Das
Anil Kumar V
Prasad Gandham
 
Publisher ICRISAT Dataverse
 
Description This dataset includes the research on the evaluation of diverse 60 genotypes including Back-Cross derived Nested Association Mapping (BCNAM), bi-parental mapping populations, and accessions from different countries such as Nigeria, USA, Lesotho, Ethiopia, and Mali for nitrogen use efficiency. DNA was isolated from 4 – 6 leaf stage of 58 Sorghum genotypes (2 genotypes did not germinate) using the modified hexadecyltrimethyl ammonium bromide (CTAB) protocol from 12-day-old seedlings. The genotyping was done following the Genotyping by Sequencing (GBS) approach with the ApeKI restriction enzyme used for complexity reduction. The Single Nucleotide Polymorphisms (SNPs) were called using TASSEL v5.2 GBS pipeline against Sorghum assembly v3. The final data included a total of 89,770 SNPs (obtained from 58 accessions out of 60) with minor allele frequency (MAF) >1% and missing data
 
Subject Agricultural Sciences
Sorghum
SNP
 
Language English
 
Contributor Deshpande, Santosh
 
Type Genotypic data