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Immunohistochemical studies on progressive pathology of ischaemia reperfusion acute kidney injury in male Sprague Dawley rats

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Title Immunohistochemical studies on progressive pathology of ischaemia reperfusion acute kidney injury in male Sprague Dawley rats
 
Creator Dubey, Amita
Prajapti, KS
Joshi, BP
Swamy, Madhu
 
Subject CD31
Vimentin
Image analysis
Chronic kidney disease
 
Description 270-274
Acute kidney injury (AKI) is a major problem in ICU patient, majority of the cases even after functional recovery ended with chronic kidney disease. In the present study, we investigated the immunohistochemical change in progressive pathology of ischaemia reperfusion induced acute kidney injury. Male Sprague Dawley (SD) rats were subjected to 35 min of renal ischemia followed by reperfusion periods of day 1, 4, 7, 14 and 28. Renal injury was confirmed by the increase serum creatinine level. The vascular and tubular changes in kidneys were studied by the CD31 and vimentin immunohistochemistry respectively. In this study, no significant changes were observed in CD31 positive cells, though lower densities of capillaries were noticed in the tubulo-interstitial lesion on day 28 of reperfusion. Significant increase in vimentin positive cells, which is a marker of undifferentiated cells, was observed on day 4 and day 7 of reperfusion. The immature cells were also present on day 28 of reperfusion indicating that these cells may would have led further progressive pathological changes in kidney even after the functional and morphological normalization.
 
Date 2021-03-31T06:17:19Z
2021-03-31T06:17:19Z
2021-04
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscair.res.in/handle/123456789/56556
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJEB Vol.59(04) [April 2021]