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<p>Overview Of  Toxic Cyanobacteria And Dinoflagellates And mcy Gene Clusters Responsible For Cyanotoxin Production</p><h2 style="text-align: left;"></h2>

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Title Statement <p>Overview Of  Toxic Cyanobacteria And Dinoflagellates And mcy Gene Clusters Responsible For Cyanotoxin Production</p><h2 style="text-align: left;"></h2>
 
Added Entry - Uncontrolled Name Lande, Vijay Wasudeorao; National Environmental Engineering Research Institute,
Environmental Impact and Sustainability Division (EISD-NEERI)
 
Uncontrolled Index Term Cyanobacteria; Dinoflagellate; Microcystin; mcyABC and mcyDE Gene Clusters; QRT-PCR Technique; MALDI-TOF-MS Analysis
 
Summary, etc. <p style="text-align: justify;"><span style="font-size: x-small;">Overview of toxic cyanobacteria  is related to cyanotoxin in this research article. Cyanobacterial bloom occur  in oligotrophic to mesotrophic conditions in waterbodies and related with environmental factors and nutrient concentrations. Analysis of microcystins, and health hazard  due to cyanotoxin of <em>Microcystis aeurignosa</em> and <em>Anabaena</em> sp.,<em> Planktothrix agardhii</em>,<em> </em>were highlighted here. It is observed that cyanobacteria, dinoflagellate toxins are of hepatotoxic and neurotoxic nature and protect cells against planktivores. Toxin from <em>Aphanizomenonflos-aquae</em>, marine dinoflagellate <em>Gonyaulax</em> sp., <em>Alexandrium</em> sp., <em>Gymnodinium</em> sp., and <em>Pyrodinium</em> sp., (saxitoxins-STX) were causing  paralytic shell fish  poisoning (in human being). Microcystins oligopeptide structure have several congeners in cyanobacteria as biotoxin  were observed several species of cyanobacteria except cylindrospermopsin,  (a guanidine alkaloid-cytotoxin). During recent years, molecular biologist’s identified  <em>mcyA</em> to<em> mcyE</em> gene clusters that synthesize microcystins in toxic cyanobacteria. Studies of degenerate primers directed to conserved functional motifs (exons – open reading frames) of <em>mcyABC</em> to <em>mcyDE</em> gene clusters. Mutational studies were conducted to locate microcystin synthetase coding gene  sequences of in <em>mcyB</em> and <em>mcyD</em> genes. Presence <em>mcyE</em> gene sequences in multiple numbers for proportionate  toxic factor of microcystins production in toxic <em>Microcystis </em>and <em>Anabaena</em> strains in lakes were reported. Microcystins inhibits eukaryotic serine/threonine protein phosphatase 1 and 2A functions and result in hepatotxic tumor promoter. Earlier researchers have studied various aspects of toxic cyanobacteria i.e. genetic markers based polymerase chain reaction (QRT-PCR), production of nonribosomally synthesis of cyclic heptapeptide of microcystins (ribosomes involve in translation process of protein synthesis) Picomolar concentration microcystin congeners can be detected using Mass M/Z (i.e. mass to charge ratio)  in Matrix Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry analysis (MALDI-TOF-MS). <em>Microsystis</em> toxin  can be destroyed in chlorine under pH dependent condition such that chlorine residual at least 0.5mg/l should be present after  contact time of 30 minute. Ozone is also preferred disinfectant for water supplies where microcystin and anatoxin may affect water quality. Microcystins causes chronic toxicity. Symptoms of  hepatotoxin toxicity are weakness, loss of appetite (anorexia), pallor of intestine mucous membranes, vomiting, cold extremities and diarrhoea. Anatoxin an irreversible inhibitor of cholinesterase.is  also known for acute toxicity e.i. staggering, muscle fasciculation gasping and convulsion.</span></p>
 
Publication, Distribution, Etc. Journal of Indian Association for Environmental Management (JIAEM)
2021-04-28 17:29:30
 
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http://op.niscair.res.in/index.php/JIAEM/article/view/43738
 
Data Source Entry Journal of Indian Association for Environmental Management (JIAEM); ##issue.vol## 41, ##issue.no## 1 (2021): Journal of Indian Association for Environmental Management (JIAEM)
 
Language Note en
 
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