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QTL Mapping of Traits Associated with Dual Resistance to the African Stem Borer (Busseola fusca) and Spotted Stem Borer (Chilo partellus) in Sorghum (Sorghum bicolor)

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Relation http://oar.icrisat.org/11791/
https://doi.org/10.1155/2021/7016712
doi:10.1155/2021/7016712
 
Title QTL Mapping of Traits Associated with Dual Resistance to the African Stem Borer (Busseola fusca) and Spotted Stem Borer (Chilo partellus) in Sorghum (Sorghum bicolor)
 
Creator Muturi, P W
Mgonja, M
Rubaihayo, P
Mwololo, J K
Ferrante, A
 
Subject Sorghum
Plant Disease
 
Description Sorghum (Sorghum bicolor (L.) Moench) is an important food crop in semi-arid tropics. The crop grain yield ranges from 0.5 t/ha to
0.8 t/ha compared to potential yields of 10 t/ha. The African stem borer Busseola fusca Fuller (Noctuidae) and the spotted stem
borer Chilo partellus Swinhoe (Crambidae), are among the most economically important insect pests of sorghum. The two
borers can cause 15% - 80% grain yield loss in sorghum. Mapping of QTLs associated with resistance traits to the two stem
borers is important towards marker-assisted breeding. The objective of this study was to map QTLs associated with resistance
traits to B. fusca and C. partellus in sorghum. 243 F9:10 sorghum RILs derived from ICSV 745 (S) and PB 15520-1 (R) were
selected for the study with 4,955 SNP markers. The RILs were evaluated in three sites. Data was collected on leaf feeding,
deadheart, exit holes, stem tunnels, leaf toughness, seedling vigour, bloom waxiness, and leaf glossiness. ANOVA for all the
traits was done using Genstat statistical software. Insect damage traits and morphological traits were correlated using Pearson’s
correlation coefficients. Genetic mapping was done using JoinMap 4 software, while QTL analysis was done using PLABQTL
software. A likelihood odds ratio (LOD) score of 3.0 was used to declare linkage. Joint analyses across borer species and sites
revealed 4 QTLs controlling deadheart formation; 6 controlling leaf feeding damage; 5 controlling exit holes and stem tunneling
damages; 2 controlling bloom waxiness, leaf glossiness, and seedling vigour; 4 conditioning trichome density; and 6 conditioning
leaf toughness. Joint analyses for B. fusca and C. partellus further revealed that marker CS132-2 colocalised for leaf toughness
and stem tunneling traits on QTLs 1 and 2, respectively; thus, the two traits can be improved using the same linked marker.
This study recommended further studies to identify gene(s) underlying the mapped QTLs.
 
Publisher Hindawi Ltd
 
Date 2021-01
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11791/1/7016712.pdf
Muturi, P W and Mgonja, M and Rubaihayo, P and Mwololo, J K and Ferrante, A (2021) QTL Mapping of Traits Associated with Dual Resistance to the African Stem Borer (Busseola fusca) and Spotted Stem Borer (Chilo partellus) in Sorghum (Sorghum bicolor). International Journal of Genomics (TSI), 2021. pp. 1-17. ISSN 2314-436X