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Physiological and Proteomic Signatures Reveal Mechanisms of Superior Drought Resilience in Pearl Millet Compared to Wheat

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Relation http://oar.icrisat.org/11795/
https://doi.org/10.3389/fpls.2020.600278
doi:10.3389/fpls.2020.600278
 
Title Physiological and Proteomic Signatures Reveal Mechanisms of Superior Drought Resilience in Pearl Millet Compared to Wheat
 
Creator Ghatak, A
Chaturvedi, P
Bachmann, G
Valledor, L
Ramšak, Z
Bazargani, M M
Bajaj, P
Jegadeesan, S
Li, W
Sun, X
Gruden, K
Varshney, R K
Weckwerth, W
 
Subject Cereals
Pearl Millet
Genetics and Genomics
Wheat
 
Description Presently, pearl millet and wheat are belonging to highly important cereal crops.
Pearl millet, however, is an under-utilized crop, despite its superior resilience to
drought and heat stress in contrast to wheat. To investigate this in more detail,
we performed comparative physiological screening and large scale proteomics of
drought stress responses in drought-tolerant and susceptible genotypes of pearl
millet and wheat. These chosen genotypes are widely used in breeding and farming
practices. The physiological responses demonstrated large differences in the regulation
of root morphology and photosynthetic machinery, revealing a stay-green phenotype
in pearl millet. Subsequent tissue-specific proteome analysis of leaves, roots and
seeds led to the identification of 12,558 proteins in pearl millet and wheat under
well-watered and stress conditions. To allow for this comparative proteome analysis
and to provide a platform for future functional proteomics studies we performed a
systematic phylogenetic analysis of all orthologues in pearl millet, wheat, foxtail millet,
sorghum, barley, brachypodium, rice, maize, Arabidopsis, and soybean. In summary, we
define (i) a stay-green proteome signature in the drought-tolerant pearl millet phenotype
and (ii) differential senescence proteome signatures in contrasting wheat phenotypes
not capable of coping with similar drought stress. These different responses have a
significant effect on yield and grain filling processes reflected by the harvest index.
Proteome signatures related to root morphology and seed yield demonstrated the
unexpected intra- and interspecies-specific biochemical plasticity for stress adaptation
 
Publisher Frontiers Media
 
Date 2021-01
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11795/1/fpls-11-600278.pdf
Ghatak, A and Chaturvedi, P and Bachmann, G and Valledor, L and Ramšak, Z and Bazargani, M M and Bajaj, P and Jegadeesan, S and Li, W and Sun, X and Gruden, K and Varshney, R K and Weckwerth, W (2021) Physiological and Proteomic Signatures Reveal Mechanisms of Superior Drought Resilience in Pearl Millet Compared to Wheat. Frontiers in Plant Science (TSI), 11. pp. 1-24. ISSN 1664-462X