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Comprehensive analysis and identification of drought-responsive candidate NAC genes in three semi-arid tropics (SAT) legume crops

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Relation http://oar.icrisat.org/11821/
https://doi.org/10.1186/s12864-021-07602-5
doi:10.1186/s12864-021-07602-5
 
Title Comprehensive analysis and identification of drought-responsive candidate NAC genes in three semi-arid tropics (SAT) legume crops
 
Creator Singh, S
Kudapa, H
Garg, V
Varshney, R K
 
Subject Drought Tolerance
Chickpea
Pigeonpea
Groundnut
Genetics and Genomics
Legume Crops
 
Description Background: Chickpea, pigeonpea, and groundnut are the primary legume crops of semi-arid tropics (SAT) and
their global productivity is severely affected by drought stress. The plant-specific NAC (NAM - no apical meristem,
ATAF - Arabidopsis transcription activation factor, and CUC - cup-shaped cotyledon) transcription factor family is
known to be involved in majority of abiotic stresses, especially in the drought stress tolerance mechanism. Despite
the knowledge available regarding NAC function, not much information is available on NAC genes in SAT legume
crops.
Results: In this study, genome-wide NAC proteins – 72, 96, and 166 have been identified from the genomes of
chickpea, pigeonpea, and groundnut, respectively, and later grouped into 10 clusters in chickpea and pigeonpea,
while 12 clusters in groundnut. Phylogeny with well-known stress-responsive NACs in Arabidopsis thaliana, Oryza
sativa (rice), Medicago truncatula, and Glycine max (soybean) enabled prediction of putative stress-responsive NACs
in chickpea (22), pigeonpea (31), and groundnut (33). Transcriptome data revealed putative stress-responsive NACs
at various developmental stages that showed differential expression patterns in the different tissues studied.
Quantitative real-time PCR (qRT-PCR) was performed to validate the expression patterns of selected stressresponsive,
Ca_NAC (Cicer arietinum - 14), Cc_NAC (Cajanus cajan - 15), and Ah_NAC (Arachis hypogaea - 14) genes
using drought-stressed and well-watered root tissues from two contrasting drought-responsive genotypes of each
of the three legumes. Based on expression analysis, Ca_06899, Ca_18090, Ca_22941, Ca_04337, Ca_04069, Ca_04233,
Ca_12660, Ca_16379, Ca_16946, and Ca_21186; Cc_26125, Cc_43030, Cc_43785, Cc_43786, Cc_22429, and Cc_22430;
Ah_ann1.G1V3KR.2, Ah_ann1.MI72XM.2, Ah_ann1.V0X4SV.1, Ah_ann1.FU1JML.2, and Ah_ann1.8AKD3R.1 were identified
as potential drought stress-responsive candidate genes.
 
Publisher BMC
 
Date 2021-04
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11821/1/s12864-021-07602-5.pdf
Singh, S and Kudapa, H and Garg, V and Varshney, R K (2021) Comprehensive analysis and identification of drought-responsive candidate NAC genes in three semi-arid tropics (SAT) legume crops. BMC Genomics (TSI), 22 (1). pp. 1-23. ISSN 1471-2164