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Comparative Transcriptome Analysis Identified Candidate Genes for Late Leaf Spot Resistance and Cause of Defoliation in Groundnut

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Relation http://oar.icrisat.org/11822/
https://doi.org/10.3390/ijms22094491
doi:10.3390/ijms22094491
 
Title Comparative Transcriptome Analysis Identified Candidate Genes for Late Leaf Spot Resistance and Cause of Defoliation in Groundnut
 
Creator Gangurde, S S
Nayak, S N
Joshi, P
Purohit, S
Sudini, H K
Chitikineni, A
Hong, Y
Guo, B
Chen, X
Pandey, M K
Varshney, R K
 
Subject Groundnut
Genetics and Genomics
Plant Disease
 
Description Late leaf spot (LLS) caused by fungus Nothopassalora personata in groundnut is responsible
for up to 50% yield loss. To dissect the complex nature of LLS resistance, comparative transcriptome
analysis was performed using resistant (GPBD 4), susceptible (TAG 24) and a resistant introgression
line (ICGV 13208) and identified a total of 12,164 and 9954 DEGs (differentially expressed genes)
respectively in A- and B-subgenomes of tetraploid groundnut. There were 135 and 136 unique
pathways triggered in A- and B-subgenomes, respectively, upon N. personata infection. Highly upregulated
putative disease resistance genes, an RPP-13 like (Aradu.P20JR) and a NBS-LRR (Aradu.Z87JB)
were identified on chromosome A02 and A03, respectively, for LLS resistance. Mildew resistance Locus
(MLOs)-like proteins, heavy metal transport proteins, and ubiquitin protein ligase showed trend
of upregulation in susceptible genotypes, while tetratricopeptide repeats (TPR), pentatricopeptide
repeat (PPR), chitinases, glutathione S-transferases, purple acid phosphatases showed upregulation
in resistant genotypes. However, the highly expressed ethylene responsive factor (ERF) and ethylene
responsive nuclear protein (ERF2), and early responsive dehydration gene (ERD) might be related to
the possible causes of defoliation in susceptible genotypes. The identified disease resistance genes
can be deployed in genomics-assisted breeding for development of LLS resistant cultivars to reduce
the yield loss in groundnut.
 
Publisher MDPI
 
Date 2021-04
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11822/1/ijms-22-04491.pdf
Gangurde, S S and Nayak, S N and Joshi, P and Purohit, S and Sudini, H K and Chitikineni, A and Hong, Y and Guo, B and Chen, X and Pandey, M K and Varshney, R K (2021) Comparative Transcriptome Analysis Identified Candidate Genes for Late Leaf Spot Resistance and Cause of Defoliation in Groundnut. International Journal of Molecular Sciences (TSI), 22 (4491). pp. 1-23. ISSN 1422-0067