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Genome sequencing and comparative genomic analysis of highly and weakly aggressive strains of Sclerotium rolfsii, the causal agent of peanut stem rot

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Relation http://oar.icrisat.org/11825/
https://doi.org/10.1186/s12864-021-07534-0
doi:10.1186/s12864-021-07534-0
 
Title Genome sequencing and comparative genomic analysis of highly and weakly aggressive strains of Sclerotium rolfsii, the causal agent of peanut stem rot
 
Creator Yan, L
Wang, Z
Song, W
Fan, P
Kang, Y
Lei, Y
Wan, L
Huai, D
Chen, Y
Wang, X
Sudini, H
Liao, B
 
Subject Genetics and Genomics
Plant Disease
 
Description Background: Stem rot caused by Sclerotium rolfsii is a very important soil-borne disease of peanut. S. rolfsii is a
necrotrophic plant pathogenic fungus with an extensive host range and worldwide distribution. It can infect
peanut stems, roots, pegs and pods, leading to varied yield losses. S. rolfsii strains GP3 and ZY collected from
peanut in different provinces of China exhibited a significant difference in aggressiveness on peanut plants by
artificial inoculation test. In this study, de-novo genome sequencing of these two distinct strains was performed
aiming to reveal the genomic basis of difference in aggressiveness.
Results: Scleotium rolfsii strains GP3 and ZY, with weak and high aggressiveness on peanut plants, exhibited similar
growth rate and oxalic acid production in laboratory. The genomes of S. rolfsii strains GP3 and ZY were sequenced
by Pacbio long read technology and exhibited 70.51 Mb and 70.61 Mb, with contigs of 27 and 23, and encoded 17,
097 and 16,743 gene models, respectively. Comparative genomic analysis revealed that the pathogenicity-related
gene repertoires, which might be associated with aggressiveness, differed between GP3 and ZY. There were 58 and
45 unique pathogen-host interaction (PHI) genes in GP3 and ZY, respectively. The ZY strain had more carbohydrateactive
enzymes (CAZymes) in its secretome than GP3, especially in the glycoside hydrolase family (GH), the
carbohydrate esterase family (CBM), and the polysaccharide lyase family (PL). GP3 and ZY also had different effector
candidates and putative secondary metabolite synthetic gene clusters. These results indicated that differences in
PHI, secreted CAZymes, effectors and secondary metabolites may play important roles in aggressive difference
between these two strains.
Conclusions: The data provided a further understanding of the S. rolfsii genome. Genomic comparison provided
clues to the difference in aggressiveness of S. rolfsii strains.
 
Publisher BMC London
 
Date 2021-04
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11825/1/s12864-021-07534-0.pdf
Yan, L and Wang, Z and Song, W and Fan, P and Kang, Y and Lei, Y and Wan, L and Huai, D and Chen, Y and Wang, X and Sudini, H and Liao, B (2021) Genome sequencing and comparative genomic analysis of highly and weakly aggressive strains of Sclerotium rolfsii, the causal agent of peanut stem rot. BMC Genomics (TSI), 22 (1). pp. 1-15. ISSN 1471-2164