Record Details

Reciprocal relation between reporter gene transcription and translation efficiency in fission yeast

DIR@IMTECH: CSIR-Institute of Microbial Technology

View Archive Info
 
 
Field Value
 
Title Reciprocal relation between reporter gene transcription and translation efficiency in fission yeast
 
Creator Srivastava, Suchita
Kaur, Satinderdeep
Verma, Hemant Kumar
Rani, Suman
Thakur, Manisha
Haldar, Swati
Singh, Jagmohan
 
Subject QR Microbiology
 
Description The fission yeast, Schizosaccharomyces pombe, is an excellent model for basic research but is not useful for commercial scale protein expression due to lack of strong expression vectors. Earlier, we showed that the lsd90 promoter elicited significantly greater GFP expression level than the adh1 and nmt1 promoters, albeit in different vector backbones. Here, we have systematically investigated the contribution of selectable markers, LEU2 and URA3m to GFP expression: while LEU2 elicited very low expression, the URA3m gene, with truncated promoter, elicited much greater GFP expression level with all promoters. Paradoxically, an inverse correlation was observed between the GFP transcription and translation efficiency. This system can be useful for understanding the factors governing recombinant gene expression and optimization of protein production.
 
Publisher Elsevier Science/Academic Press
 
Date 2021-05
 
Type Article
PeerReviewed
 
Relation https://www.sciencedirect.com/science/article/abs/pii/S0147619X21000044?via%3Dihub
http://crdd.osdd.net/open/2697/
 
Identifier Srivastava, Suchita and Kaur, Satinderdeep and Verma, Hemant Kumar and Rani, Suman and Thakur, Manisha and Haldar, Swati and Singh, Jagmohan (2021) Reciprocal relation between reporter gene transcription and translation efficiency in fission yeast. Plasmid, 115. ISSN 1095-9890