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<p><strong>Interferon gamma and IP 10 mRNA quantitative real time PCR in whole blood culture of guinea pig and cattle to multi-antigen recombinant protein cocktail and PPD of <em>Mycobacterium bovis </em>(3/86Rv)</strong></p>

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Title Statement <p><strong>Interferon gamma and IP 10 mRNA quantitative real time PCR in whole blood culture of guinea pig and cattle to multi-antigen recombinant protein cocktail and PPD of <em>Mycobacterium bovis </em>(3/86Rv)</strong></p>
 
Added Entry - Uncontrolled Name Ramane, Sangram
Verma, Rishendra
 
Uncontrolled Index Term Bovine tuberculosis, Cattle, Livestock, Piggery
 
Summary, etc. <p class="Abstract" style="text-align: justify;"><em>In vivo</em> tuberculin skin test and <em>in vitro</em> IFNγ assays are the most explored diagnostics for <em>Mycobacterium bovis</em> infection (Bovine Tuberculosis) in animals. However, there are other potential biomarkers like IP10 which may allow diagnosis of tuberculosis. In this study, IFNγ and IP10 mRNA responses in guinea pig and cattle were determined by quantitative RT-PCR in whole blood samples stimulated PPDs and recombinant protein cocktail containing eight purified rCFP2, rESAT6, rCFP10, rMTC28, rMPB53, rMPB63, rMPB64 and rMPB83 proteins. Blood samples were obtained from <em>M. bovis</em> sensitised guinea pigs and tuberculin test positive cattle. Both guinea pigs and cattle blood cultures produced a significant increase in mRNA level of IFNγ and IP10 when stimulated with protein cocktail as compared to that of bovine PPD. Upon different antigenic stimulations, IP10 mRNA responses were followed the similar kinetics to those of IFNγ with high correlation (r = 0.97 for guinea pig and 0.96 for cattle).</p>
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2021-06-30 15:40:35
 
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http://op.niscair.res.in/index.php/IJEB/article/view/51873
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 59, ##issue.no## 06 (2021): IJEB [JUNE 2021]
 
Language Note en