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<p><strong>Statistical optimization of alkaline protease from <em>Bacillus amyloliquefaciens </em>SP1</strong></p>

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Title Statement <p><strong>Statistical optimization of alkaline protease from <em>Bacillus amyloliquefaciens </em>SP1</strong></p>
 
Added Entry - Uncontrolled Name Sharma, Shiwani Guleria
Walia, Abhishek
Chauhan, Anjali
Shirkot, C K
 
Uncontrolled Index Term Apple rhizosphere, Central composite design, Placket Burmann design, Protease, Response surface methodology (RSM)
 
Summary, etc. <p style="text-align: justify;">Alkaline protease has tremendous applications in different industries, and to fulfil industrial and commercial enzyme requirements, overproduction and optimization of production medium are prerequisites. Protease production by <em>Bacillus amyloliquefaciens </em>SP1, isolated from apple tree rhizosphere was studied. Plackett Burmann design<strong> </strong>and response surface methodology was used to optimize medium for alkaline protease production. Higher levels of protease activity were observed in presence of disaccharides owing to diauxic growth and inorganic nitrogen sources proved to be less favourable. After screening through one-factor-at-a time experiments and Plackett Burmann design, casein, yeast extract, maltose and KH<sub>2</sub>PO<sub>4 </sub>were identified as the most significant variables. Statistical design based three-dimensional (3-D) and contour plots were generated to understand the relationship between enzyme activity and medium variables. The protease production was found to increase from 1730 to 3630<strong> </strong>µg/ml/min in optimized medium i.e. approx. 2.1 fold increased compared with original medium. The study assumes significance in the ability of bacterium to survive in wide variety of salts and agricultural wastes and yield optimum levels of extracellular protease.</p>
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2021-06-30 15:40:35
 
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http://op.niscair.res.in/index.php/IJEB/article/view/51874
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 59, ##issue.no## 06 (2021): IJEB [JUNE 2021]
 
Language Note en