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<p><strong>Hepatocyte nuclear factor Foxa-2 effectively differentiates mesenchymal stem cells to functional hepatocytes</strong></p>

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Title Statement <p><strong>Hepatocyte nuclear factor Foxa-2 effectively differentiates mesenchymal stem cells to functional hepatocytes</strong></p>
 
Added Entry - Uncontrolled Name Dhanasekaran, Sugapriya ; Department of Medical Laboratory Sciences (Pathology), College of Applied Medical Sciences, Prince Sattam Bin Abdul-Aziz University, Wadi Ad Dawaser Campus-11991, P.O. Box 54, Riyadh province, Saudi Arabia
Sithambaram, Devilakshmi ; Indian Institute of Technology Madras
Verma, Rama S; Indian Institute of Technology Madras
 
Uncontrolled Index Term Cytochrome P450, Hepatocyte nuclear factor,, Liver development differentiation, Nuclear transcription factor
 
Summary, etc. <p style="text-align: justify;">Overexpression of hepatocyte nuclear factor (Foxa-2), a master regulator significantly enhances the hepatic differentiation by triggering targeted liver-specific gene expression for liver development. We investigated the function of key regulators that facilitate hepatic differentiation. The functional hepatocyte was determined by observing morphological changes, expression of liver specific markers through immunocytochemical analysis, qRT-PCR, Western Blot and specific liver function assays. Our study confirmed that induction of functional hepatocyte shows typical epithelial morphology, express hepatic genes and acquire hepatocyte functions. Notably, these cells expressed the markers of mature hepatocytes, including albumin, tyrosine aminotransferase, cytokeratin, Cyp7A1 and hepatic transcription factors. Furthermore, these cells exhibited hepatic functions <em>in vitro</em>, including glycogen storage, albumin production, urea secretion and functional assessment of hepatocytes. We have developed a robust and efficient method to differentiate mesenchymal stem cells into induced functional hepatocytes, which exhibits characteristics of mouse hepatocytes. Apparently, induction of functional hepatocytes will pave a better cellular therapy or effective cell source within a short timeline to facilitate the development of hepatocytes for future clinical applications of regenerative medicine.</p>
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2021-06-30 15:40:35
 
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http://op.niscair.res.in/index.php/IJEB/article/view/51862
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 59, ##issue.no## 06 (2021): IJEB [JUNE 2021]
 
Language Note en