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Phenotypic and molecular typing of Vibrio harveyi isolates and their pathogenicity to tiger shrimp larvae

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Relation http://eprints.cmfri.org.in/6835/
 
Title Phenotypic and molecular typing of Vibrio harveyi isolates and their pathogenicity to tiger shrimp larvae
 
Creator Alavandi, S V
Manoranjita, V
Vijayan, K K
Kalaimani, N
Santiago, T C
 
Subject Eggs and Larvae
Prawn and Prawn fisheries
 
Description The objective of the present study was to identify the biotype(s) and
molecular type(s) of Vibrio harveyi associated with pathogenicity in tiger
shrimp (Penaeus monodon) larvae.
Five luminescent and four nonluminescent V. harveyi
isolates were subjected to phenotyping and random amplified polymorphic
DNA (RAPD) fingerprinting, and pathogenicity testing to P. monodon mysis.
Four isolates induced 34–41% mortality of P. monodon mysis when challenged
at the rate of 106 CFU ml)1 within 60 h. Sucrose-fermenting biotypes of
V. harveyi appeared to be associated with pathogenicity to larval shrimp.
Higher temperature and salinity appeared to play a role on the onset of vibriosis
and mortality in the challenged larval shrimp. Pathogenic isolates of
V. harveyi could be demarcated as revealed by their clustering in the dendrogram
constructed based on the RAPD fingerprints.
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/6835/1/kkv_17V_harveyiLtApMicrobiol2006.pdf
Alavandi, S V and Manoranjita, V and Vijayan, K K and Kalaimani, N and Santiago, T C (2006) Phenotypic and molecular typing of Vibrio harveyi isolates and their pathogenicity to tiger shrimp larvae. Letters in Applied Microbiology, 43. pp. 566-570.