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Nitrogen Use Efficiency in Sorghum: Exploring Native Variability for Traits Under Variable N-Regimes

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Relation http://oar.icrisat.org/11857/
https://doi.org/10.3389/fpls.2021.643192
doi:10.3389/fpls.2021.643192
 
Title Nitrogen Use Efficiency in Sorghum: Exploring Native Variability for Traits Under Variable N-Regimes
 
Creator Bollam, S
Romana, K K
Rayaprolu, L
Anil kumar, V
Das, R R
Rathore, A
Gandham, P
Chander, G
Deshpande, S P
Gupta, R
 
Subject Sorghum
Genetics and Genomics
 
Description Exploring the natural genetic variability and its exploitation for improved Nitrogen
Use Efficiency (NUE) in sorghum is one of the primary goals in the modern crop
improvement programs. The integrated strategies include high-throughput phenotyping,
next generation sequencing (NGS)-based genotyping technologies, and a priori selected
candidate gene studies that help understand the detailed physiological and molecular
mechanisms underpinning this complex trait. A set of sixty diverse sorghum genotypes
was evaluated for different vegetative, reproductive, and yield traits related to NUE in
the field (under three N regimes) for two seasons. Significant variations for different
yield and related traits under 0 and 50% N confirmed the availability of native
genetic variability in sorghum under low N regimes. Sorghum genotypes with distinct
genetic background had interestingly similar NUE associated traits. The Genotyping-
By-Sequencing based SNPs (>89 K) were used to study the population structure,
and phylogenetic groupings identified three distinct groups. The information of grain N
and stalk N content of the individuals covered on the phylogenetic groups indicated
randomness in the distribution for adaptation under variable N regimes. This study
identified promising sorghum genotypes with consistent performance under varying
environments, with buffer capacity for yield under low N conditions. We also report
better performing genotypes for varied production use—grain, stover, and dual-purpose
sorghum having differential adaptation response to NUE traits. Expression profiling
of NUE associated genes in shoot and root tissues of contrasting lines (PVK801
and HDW703) grown in varying N conditions revealed interesting outcomes. Root
tissues of contrasting lines exhibited differential expression profiles for transporter genes
[ammonium transporter (SbAMT), nitrate transporters (SbNRT)]; primary assimilatory
(glutamine synthetase (SbGS), glutamate synthase (SbGOGAT[NADH], SbGOGAT[Fd]),
assimilatory genes [nitrite reductase (SbNiR[NADH]3)]; and amino acid biosynthesis
associated gene [glutamate dehydrogenase (SbGDH)]. Identification and expression profiling of contrasting sorghum genotypes in varying N dosages will provide new
information to understand the response of NUE genes toward adaptation to the
differential N regimes in sorghum. High NUE genotypes identified from this study could
be potential candidates for in-depth molecular analysis and contribute toward the
development of N efficient sorghum cultivars.
 
Publisher Frontiers Media
 
Date 2021-04
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11857/1/fpls-12-643192.pdf
Bollam, S and Romana, K K and Rayaprolu, L and Anil kumar, V and Das, R R and Rathore, A and Gandham, P and Chander, G and Deshpande, S P and Gupta, R (2021) Nitrogen Use Efficiency in Sorghum: Exploring Native Variability for Traits Under Variable N-Regimes. Frontiers in Plant Science (TSI), 12 (643192). pp. 1-19. ISSN 1664-462X