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Cryopreservation of spermatophores of the marine shrimp Penaeus indicus H. Milne Edwards

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Relation http://eprints.cmfri.org.in/7896/
 
Title Cryopreservation of spermatophores of the marine shrimp Penaeus indicus H. Milne Edwards
 
Creator Diwan, A D
Joseph, Shoji
 
Subject Prawn and Prawn fisheries
 
Description Attempts on cryopreservation of spermatozoa have been made in penaeid shrimp Penaeus indicus. It was observed that viable spermatozoa could bepreserved successfully for a period of60 days at -35"C and -196"C temperature. Among several cryoprotectantsused a combination of DMSO (5%) + glycerol (5%) and DMSO (5%) + trehalose (0.25 M) showed best viability rate (75 to 80%) in freeze thawed spermatoza. The activational changes in spermatozoa during acrosome reaction after induction with anegg water have also been mentioned. The importance
of cryogenic storage of spermatozoa has been discussed.
 
Publisher Nature Conservators
 
Contributor Ponniah, A G
Das, P
Verma, S R
 
Date 1998
 
Type Book Section
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/7896/1/B152-FISH_GENETICS_AND_BIODIVERSITY_CONSERVATION_A.D.DIWAN1998..pdf
Diwan, A D and Joseph, Shoji (1998) Cryopreservation of spermatophores of the marine shrimp Penaeus indicus H. Milne Edwards. In: Fish Genetics and Biodiversity Conservation. Nature Conservators, Mussafarnagar, pp. 243-251.