Cryopreservation of spermatophores of the marine shrimp Penaeus indicus H. Milne Edwards
CMFRI Repository
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Relation |
http://eprints.cmfri.org.in/7896/
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Title |
Cryopreservation of spermatophores of the marine shrimp Penaeus indicus H. Milne Edwards
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Creator |
Diwan, A D
Joseph, Shoji |
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Subject |
Prawn and Prawn fisheries
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Description |
Attempts on cryopreservation of spermatozoa have been made in penaeid shrimp Penaeus indicus. It was observed that viable spermatozoa could bepreserved successfully for a period of60 days at -35"C and -196"C temperature. Among several cryoprotectantsused a combination of DMSO (5%) + glycerol (5%) and DMSO (5%) + trehalose (0.25 M) showed best viability rate (75 to 80%) in freeze thawed spermatoza. The activational changes in spermatozoa during acrosome reaction after induction with anegg water have also been mentioned. The importance of cryogenic storage of spermatozoa has been discussed. |
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Publisher |
Nature Conservators
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Contributor |
Ponniah, A G
Das, P Verma, S R |
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Date |
1998
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Type |
Book Section
PeerReviewed |
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Format |
application/pdf
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Language |
en
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Identifier |
http://eprints.cmfri.org.in/7896/1/B152-FISH_GENETICS_AND_BIODIVERSITY_CONSERVATION_A.D.DIWAN1998..pdf
Diwan, A D and Joseph, Shoji (1998) Cryopreservation of spermatophores of the marine shrimp Penaeus indicus H. Milne Edwards. In: Fish Genetics and Biodiversity Conservation. Nature Conservators, Mussafarnagar, pp. 243-251. |
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