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Isolation and Characterization of Bacteria associated with Cultured Penaeus monodon affected by Loose Shell Syndrome

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Relation http://eprints.cmfri.org.in/8971/
 
Title Isolation and Characterization of Bacteria associated with Cultured Penaeus monodon affected by Loose Shell Syndrome
 
Creator Loka, Jayasree
Janakiram, P
Madhavi, R
 
Subject Microbiology
Farming/Culture
Prawn and Prawn fisheries
 
Description Seventy shrimps affected by loose shell syndrome (LSS), a major disease causing mass mortalities in shrimp farms in north coastal Andhra Pradesh (India), were subjected to bacteriological analysis. Total bacteria and vibrio counts in the hemolymph were estimated; bacteria were isolated and identified; their pathogenicity and drug sensitivity were studied. Total bacteriaranged 1.28 x 102 to 5.8 x 105 cfu/ml while Vibrioranged 0.7 x 102to 2.1 x 105 cfu/ml. Six speciesof Vibriowere isolated from the hemolymph including V. harveyi, V. alginolyticus, V. para-haemolyticus, V. anguillarum, V. splendidus, and V. vulnificus. Vibrio harveyiwas the most dom-inant with a prevalence of 100%. It was also the most virulent with a low LC50 value of 1 x 103cfu/g. Histopathological study revealed changes typical of bacterial septicemia including invasion of bacteria into the hemolymph, formation of granulomas in the hepatopancreas and other organs, and extensive proliferation of connective tissue and necrosis of the hepatopancreas,gills, and ovarian tissues. Sensitivity to 22 antibiotics was tested. Most of the bacterial isolateswere highly sensitive to chloramphenicol, ciprofloxacin, and norfloxacin while V. harveyi was resistant to as many as 16 antibiotic drugs including chloramphenicol
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/8971/1/IJA_V.60.1_2008_46-56.pdf
Loka, Jayasree and Janakiram, P and Madhavi, R (2008) Isolation and Characterization of Bacteria associated with Cultured Penaeus monodon affected by Loose Shell Syndrome. The Israeli Journal of Aquaculture – Bamidgeh, 60 (1). pp. 46-56.