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Effect of constant light on the morphology of the retina in domestic chick (Gallus g. domesticus): A scanning electron microscope study

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Title Statement Effect of constant light on the morphology of the retina in domestic chick (Gallus g. domesticus): A scanning electron microscope study
 
Added Entry - Uncontrolled Name Gorla, Shilpa
Nag, Tapas C
 
Uncontrolled Index Term Chick retina; Cones; Constant light; Light damage; Rods; SEM
 
Summary, etc. Light induces variable levels of damage to the retina and often, death of the photoreceptor cells. It is reported that chick retina, havingabundant melanin in the retinal pigment epithelium, undergoes no or minimal damage from exposure to light. However, the morphology of cone and rod photoreceptor cells after continuous exposure of light has not been studied in detail. The present study investigated the detailed changes of photoreceptor cells, by scanning electron microscopy (SEM).Domestic chicks (Gallus g. domesticus) from post-hatch day (PH) 1 to 7 were initially reared at 700 lux at 12 h light (L): 12 h dark (D). From PH 7 to 18, they were exposed to 12L: 12D (control) and 24L: 0D (constant light) cycles at 700 lux. At PH18, chicks were sacrificed, their eyes enucleated and preserved in Karnovsky’s fixative. Theretina was then separated from the adherent choroid, mounted on photoreceptor side up and processed for SEM. There was significant damage to rod and cone outer segmentsfrom both dorsal and ventral part of the retina and decrease in the abundance of cells of inner nuclear layer in constant light, compared to that found in 12L: 12D cycles. It is likely that constant light affects the retinal cells in the vertical pathway, which could alter the photo transduction process.
 
Publication, Distribution, Etc. Indian Journal of Biochemistry and Biophysics (IJBB)
2021-07-27 16:47:04
 
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http://op.niscair.res.in/index.php/IJBB/article/view/51478
 
Data Source Entry Indian Journal of Biochemistry and Biophysics (IJBB); IJBB Vol. 58 (4) [August 2021]
 
Language Note en