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Effect of constant light on the morphology of the retina in domestic chick (Gallus g. domesticus): A scanning electron microscope study

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Title Effect of constant light on the morphology of the retina in domestic chick (Gallus g. domesticus): A scanning electron microscope study
 
Creator Gorla, Shilpa
Nag, Tapas C
 
Subject Chick retina
Cones
Ganglion cell layer (GCL)
Outer plexiform layer
Rods
Vertebrate retina
 
Description 381-384
Light induces variable levels of damage to the retina and often, death of the photoreceptor cells. It is reported that chick
retina, having abundant melanin in the retinal pigment epithelium, undergoes no or minimal damage from exposure to light.
However, the morphology of cone and rod photoreceptor cells after continuous exposure of light has not been studied in
detail. The present study investigated the detailed changes of photoreceptor cells, by scanning electron microscopy (SEM).
Domestic chicks (Gallus g. domesticus) from post-hatch day (PH) 1 to 7 were initially reared at 700 lux at 12 h light (L):12
h dark (D). From PH 7 to 18, they were exposed to 12L:12D (control) and 24L:0D (constant light) cycles at
700 lux. At PH18, chicks were sacrificed, their eyes enucleated and preserved in Karnovsky’s fixative. The retina was then
separated from the adherent choroid, mounted on photoreceptor side up and processed for SEM. There was significant
damage to rod and cone outer segments from both dorsal and ventral part of the retina and a decrease in the abundance of
cells of the inner nuclear layer in constant light, compared to that found in 12L:12D cycles. It is likely that constant light
affects the retinal cells in the vertical pathway, which could alter the phototransduction process.
 
Date 2021-07-29T11:32:58Z
2021-07-29T11:32:58Z
2021-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscair.res.in/handle/123456789/57780
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBB Vol.58(4) [August 2021]