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Ameliorating potentials of N-acetyl-l-cysteine against methoxychlor instigated modulation in structural characteristics of granulosa cells of caprine antral follicles

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Title Ameliorating potentials of N-acetyl-l-cysteine against methoxychlor instigated modulation in structural characteristics of granulosa cells of caprine antral follicles
 
Creator Bhardwaj, Jitender Kumar
Saraf, Priyanka
 
Subject Folliculogenesis
Granulosa Cells
Methoxychlor,
N-acetyl-l-cysteine
Steroidogenesis
 
Description 366-372
Follicular granulosa cells (GCs) form an important association with follicle’s survival and health that determines
ovulation. Apoptosis induction in GCs leads to follicular atresia and infertility problems. The present study aims at assessing
the ultrastructural toxicity of methoxychlor (MXC), an organochlorine insecticide and reproductive toxin, on structural
aspects of GCs owing to its potential for inducing apoptosis and the ameliorating potential of N-acetyl-L-cysteine (NAC) in
this toxicity. The ultrastructural morphology revealed MXC induced degenerative changes in GCs like loss of cellular
junction complexes and membrane integrity; nuclear abnormalities like occurrence of condensed and marginated chromatin,
crescent shaped or blebbed nucleus; presence of disrupted mitochondria with disrupted cristae, vacuolization, empty spaces,
large number of homogenous lipid droplets and extensive network of rough endoplasmic reticulum and several cytoplasmic
processes at various doses of MXC with maximum degeneration observed at 100 μg/ml. NAC supplementation reduced the
observed apoptotic characteristics of GCs with most significant changes at 5- and 10 mM concentration.
Thus, it is evident that MXC acts as an apoptotic inducer in GCs that influences the quality of antral follicles in
mammals; however, NAC, with its anti-oxidative and anti-inflammatory properties, turns out to be a potential therapeutic
and anti-apoptotic agent against MXC toxicity.
 
Date 2021-07-29T11:38:28Z
2021-07-29T11:38:28Z
2021-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscair.res.in/handle/123456789/57782
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBB Vol.58(4) [August 2021]