<em>In vitro</em> hepatoprotective activity of <em>Eichhornia Crassipes </em>flowers against CCl<sub>4</sub> induced toxicity in BRL3A cell line
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Title Statement |
<em>In vitro</em> hepatoprotective activity of <em>Eichhornia Crassipes </em>flowers against CCl<sub>4</sub> induced toxicity in BRL3A cell line |
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Added Entry - Uncontrolled Name |
M, Prasanth Kumar; Vignan Pharmacy College, Vadlamudi 522213, Guntur (Dt.), Andhra Pradesh, India V, Suba ; Department of Pharmacology, National Institute of Siddha, Chennai-600047, Tamil Nadu, India B, Rami Reddy; Natco Pharma Limited, Hyderabad 500034, Telangana, India P, Srinivasa Babu; Vignan Pharmacy College, Vadlamudi 522213, Guntur (Dt.), Andhra Pradesh, India |
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Uncontrolled Index Term |
BRL3A cell line; CCl4; Eichhornia crassipes; Hepatoprotective. |
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Summary, etc. |
<p style="text-align: justify;">The present study was carried out to determine the <em>in vitro</em> hepatoprotective activity of ethanolic extract from <em>Eichhornia crassipes </em>(EEEC) flowers using the CCl<sub>4</sub>-challenged BRL3A cell model. Hepatoprotective activity of EEEC (at concentrations of 50, 100 and 200 μg/mL) and standard drug silymarin (200 μg/mL) was evaluated against CCl<sub>4</sub> induced toxicity using BRL3A cell line by measuring the cell viability, aspartate aminotransferase (AST), alanine aminotransaminase (ALT), lactate dehydrogenase (LDH) leakage, lipid peroxidation (LPO) and glutathione level (GSH). Treatment with CCl<sub>4 </sub>produced a significant decrease in cell viability. In addition, hepatotoxicity was revealed by increased hepatic marker enzymes like AST, ALT and LDH paralleled with elevated lipid peroxidation and decline in GSH levels. The toxicity induced by CCl<sub>4</sub> in the BRL3A cells was significantly recovered by treatment with EEEC. The tested doses (100 and 200 μg/mL) significantly (<em>P</em> <0.01) reduced the CCl<sub>4</sub> induced elevation of AST, ALT and LDH and also restored the altered biochemical parameters. These findings provide a basis for confirming the traditional uses of <em>E. crassipes</em> in treating liver ailments.</p> |
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Publication, Distribution, Etc. |
Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)] 2021-08-02 14:11:01 |
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Electronic Location and Access |
application/pdf http://op.niscair.res.in/index.php/IJNPR/article/view/40389 |
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Data Source Entry |
Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]; ##issue.vol## 12, ##issue.no## 2 (2021): June 2021 |
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Language Note |
en |
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Terms Governing Use and Reproduction Note |
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