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<p><strong><em>In vitro </em>florigenesis: an efficient regeneration system avoiding time consuming vegetative phase in popular Indian soybean variety JS-335</strong></p>

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Title Statement <p><strong><em>In vitro </em>florigenesis: an efficient regeneration system avoiding time consuming vegetative phase in popular Indian soybean variety JS-335</strong></p>
 
Added Entry - Uncontrolled Name Kurumwanshi, Rashmi Shamkumar; Biotechnology Centre, Post Graduate Institute, Dr. Panjabrao Deshmukh Krishi Vidyapeeth (State Agriculture University), Akola - 444 104, Maharashtra, India
Jadhav, Pravin Vishwanathrao; Biotechnology Centre, Post Graduate Institute, Dr. Panjabrao Deshmukh Krishi Vidyapeeth (State Agriculture University), Akola - 444 104, Maharashtra, India
Surbhaiyya, Shobha Devidas; Biotechnology Centre, Post Graduate Institute, Dr. Panjabrao Deshmukh Krishi Vidyapeeth (State Agriculture University), Akola - 444 104, Maharashtra, India
Moharil, Mangesh Pradip; Biotechnology Centre, Post Graduate Institute, Dr. Panjabrao Deshmukh Krishi Vidyapeeth (State Agriculture University), Akola - 444 104, Maharashtra, India
Nandanwar, Ravindra Shamrao; Department of Agricultural Botany, Post Graduate Institute, Dr. Panjabrao Deshmukh Krishi Vidyapeeth (State Agriculture University), Akola - 444 104, Maharashtra, India
Wandhare, Madan Ramkrushna; Department of Agricultural Botany, Post Graduate Institute, Dr. Panjabrao Deshmukh Krishi Vidyapeeth (State Agriculture University), Akola - 444 104, Maharashtra, India
Manjaya, Joy Gilbart; Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre (BARC), Trombay, Mumbai - 400 085, Maharashtra, India
 
Uncontrolled Index Term Flower induction medium, Glycine max, In vitro regeneration, In vitro seed development
 
Summary, etc. Soybean (<em>Glycine max</em> L.) is a major legume crop cultivated principally as protein rich bean. Improving quality and yield received considerable attention from researchers. Here, we explored molecular approaches to improve soybean quality and yield. In spite of recalcitrance, soybean plants successfully regenerated through complex and time consuming <em>in vitro</em> regeneration protocols via organogenesis and/or somatic embryogenesis and being used for transgenic development. Transformation efficiency is highly dependent on the regeneration as not all the cells transformed lead to the recovery of viable plant regeneration. Consequently, efficient <em>in vitro</em> regeneration found to be directly associated with the recovery of transformants. In the present study, we standardized the <em>in vitro </em>florigenesis using cotyledonary node with axillary bud as explant of soybean variety JS-335. Flower buds were directly induced from proximal end of the explant on Murashige-Skoog (MS) medium augmented with thidiazuron (TDZ) and α naphthalene acetic acid (NAA). TDZ proved a potential growth regulator to induce <em>in vitro </em>florigenesis. As a result of <em>in vitro</em> fertilization, pods were developed from flowers and matured within 40-45 days on hormone-free medium. Pods and seed set under <em>in vitro </em>conditions resemble pods and seeds produced under <em>in vivo </em>conditions. This pathway of <em>in vitro </em>florigenesis showed great potential for successful induction of <em>in vitro </em>flowering, which in turn can be explored in producing transgenic soybean seeds in popular Indian soybean genotype without escaping transgene.
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2021-08-02 15:25:09
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/IJEB/article/view/53156
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 59, ##issue.no## 7 (2021): IJEB [JULY 2021]
 
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