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<p class="Default"><strong>Novel application of <em>Nerium </em>leaf and Image J software in drop collapse assay for rapid screening of biosurfactant producing microorganisms</strong></p>

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Title Statement <p class="Default"><strong>Novel application of <em>Nerium </em>leaf and Image J software in drop collapse assay for rapid screening of biosurfactant producing microorganisms</strong></p>
 
Added Entry - Uncontrolled Name Mishra, Neelam ; A DBT Research laboratory, Department of Microbiology, Gulbarga University, Kalaburagi - 585 106, Karnataka, India
Agsar, Dayanand ; A DBT Research laboratory, Department of Microbiology, Gulbarga University, Kalaburagi - 585 106, Karnataka, India
Deepthi, Seelam Siva; A DBT Research laboratory, Department of Microbiology, Gulbarga University, Kalaburagi - 585 106, Karnataka, India
Kattegouda, Umesh Madire; A DBT Research laboratory, Department of Microbiology, Gulbarga University, Kalaburagi - 585 106, Karnataka, India
Shetty, Prakasham Reddy; Bioengineering and Environmental Centre, CSIR-Indian Institute of Chemical Technology, Hyderabad - 500 007, Telangana, India
Pandey, Vijyendra ; School of Social and Behavioural Sciences, Central University of Karnataka, Kadaganchi - 585 367, Karnataka, India
 
Uncontrolled Index Term Cutin layer, Contact angle, Drop diameter, Critical micellar concentration
 
Summary, etc. <p style="text-align: justify;">Biosurfactants are attractive molecules with varied applicationsmainly oil degradation, emulsification, bioremediation, therapeutics and conjugation of nanoparticles. The existing screening methods for biosurfactants are inappropriate and too tedious. Here, we have explored a novel approach with drop collapse assay wherein we replaced the microtiter well plate with the naturally hydrophobic <em>Nerium </em>(<em>Nerium oleander </em>L<em>.</em>) leaf. The stability of beaded drops on the leaf indicates negative phenomenon, and spreading of drop indicates positive phenomenon for surfactant property, as confirmed by the measuring drop diameter using Image J software. Fifty five bacterial cultures isolated from oil contaminated site were screened through this novel approach which revealed that the isolates DNM49 (6.75±0.29 mm), DNM50 (7.45±0.19 mm) and DNM51 (6.14±0.82 mm) were the best in terms of surface tension reduction, although thirty other isolates were also found to be positive. A gradation of activity in terms of surface tension reduction was also established based on drop diameter. The results demonstrated promising application of <em>Nerium </em>leaf with Image J software in drop collapse assay as an eco-friendly and cost-effective and technically authenticated alternative to the existing assays.</p>
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2021-08-02 15:25:09
 
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http://op.niscair.res.in/index.php/IJEB/article/view/53159
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 59, ##issue.no## 7 (2021): IJEB [JULY 2021]
 
Language Note en