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<p><strong>Apoptosis mediated cytotoxic potential of <em>Erythrina variegata</em> L. stem bark in human breast carcinoma cell lines</strong></p>

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Title Statement <p><strong>Apoptosis mediated cytotoxic potential of <em>Erythrina variegata</em> L. stem bark in human breast carcinoma cell lines</strong></p>
 
Added Entry - Uncontrolled Name John, Reni ; Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences (Kerala Veterinary and Animal Sciences University), Mannuthy, Thrissur, Kerala
Kariyil, Bibu John; Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences (Kerala Veterinary and Animal Sciences University), Mannuthy, Thrissur, Kerala
PTA, Usha ; Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences (Kerala Veterinary and Animal Sciences University), Mannuthy, Thrissur, Kerala
S, Surya ; Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences (Kerala Veterinary and Animal Sciences University), Mannuthy, Thrissur, Kerala
 
Uncontrolled Index Term Anticancer, Antitumour, B-cell lymphoma-2, GC-HRMS, Indian coral tree, Mitochondria
 
Summary, etc. <p class="Abstract" style="text-align: justify;"><em>Erythrina variegata</em> L. (Fam. Fabaceae) is traditionally known for antitumour, expectorant, febrifuge, antibacterial and antioxidant activities.<em> </em>In the present study, we did cytotoxic evaluation of the methanol extract of <em>E. variegata</em> stem bark (MEV) using 3-(4,5-dimethyl thazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay in MCF-7 and MDA-MB-231 human breast carcinoma cell lines. The cytological and metabolic alterations due to MEV was evaluated using acridine orange/ethidium bromide<em> </em>(AO/EB) dual staining, hoechst 33258 and fluoroprobe, benzimidazol-carbocyanine iodide 5,5',6,6'-tetrachloro-1,1',3,3'-tetra ethyl (JC-1) staining. The antiapoptotic gene and protein, Bcl-2 expression was analysed using real time PCR and Western blotting, respectively. The methanol extract was investigated for the presence of various phytochemicals using gas chromatography high resolution mass spectrometry (GC-HRMS). The results revealed a dose dependent cytotoxic activity in both MDA-MB-231 and MCF-7 breast carcinoma cell lines <em>in vitro</em>. The plant extract was effective in inducing apoptotic cell death via mitochondrial dependent intrinsic pathway and may be considered as a potent source for isolating therapeutic molecules against cancer.</p>
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2021-08-02 15:25:09
 
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http://op.niscair.res.in/index.php/IJEB/article/view/53151
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 59, ##issue.no## 7 (2021): IJEB [JULY 2021]
 
Language Note en