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<p class="Default"><strong>Changes in expression of polyamines and ethylene biosynthesis genes in groundnut (<em>Arachis hypogaea </em>L.) genotypes during <em>Sclerotium rolfsii</em> infection</strong></p>

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Title Statement <p class="Default"><strong>Changes in expression of polyamines and ethylene biosynthesis genes in groundnut (<em>Arachis hypogaea </em>L.) genotypes during <em>Sclerotium rolfsii</em> infection</strong></p>
 
Added Entry - Uncontrolled Name Nogiya, Rakesh ; Department of Biotechnology and Biochemistry, College of Agriculture, Junagadh Agricultural University, Junagadh-362 001, Gujarat, India
Mahatma, M K; ICAR-Directorate of Groundnut Research, Post Box No. 5, Junagadh-362 001, Gujarat, India
H, Avinash Gowda; Department of Biotechnology and Biochemistry, College of Agriculture, Junagadh Agricultural University, Junagadh-362 001, Gujarat, India
Raval, S S; Department of Biotechnology and Biochemistry, College of Agriculture, Junagadh Agricultural University, Junagadh-362 001, Gujarat, India
Rathod, V ; Department of Biotechnology and Biochemistry, College of Agriculture, Junagadh Agricultural University, Junagadh-362 001, Gujarat, India
Gajera, H P; Department of Biotechnology and Biochemistry, College of Agriculture, Junagadh Agricultural University, Junagadh-362 001, Gujarat, India
Tomar, R S; Department of Biotechnology and Biochemistry, College of Agriculture, Junagadh Agricultural University, Junagadh-362 001, Gujarat, India
Kumar, Narendra ; ICAR-Directorate of Groundnut Research, Post Box No. 5, Junagadh-362 001, Gujarat, India
 
Uncontrolled Index Term Fungal, host-pathogen interaction, Stem rot disease
 
Summary, etc. <p class="Default" style="text-align: justify;">Stem rot disease caused by fungal pathogen, <em>Sclerotium rolfsii</em> Sacc., is potential threat to groundnut production in warm and humid condition. After host-pathogen interaction, a multitude of plant resistance associated reactions are initiated. In the present investigation we studied the role of polyamines and ethylene during host-pathogen interaction in stem rot tolerant (CS319, GG17 and GG31) and susceptible (TG37A) groundnut genotypes at 24, 48 and 72 h after infection. Stem rot tolerant genotypes showed higher expression of polyamine biosynthesis genes ornithine decarboxylase (<em>Ordec</em>),<em> </em>spermine synthase (<em>Sms</em>)<em> </em>and lipoxygenase1 (<em>LOX1</em>) gene at 72 h after infection than that of susceptible genotype TG37A. The expression analysis of ethylene biosynthesis genes (1-aminocyclopropane-1-carboxylate oxidase: <em>ACCO </em>and (<em>ACCS</em>) showed up regulation in stem rot susceptible genotype TG37A than that of tolerant genotypes after infection at all stages (24, 48 and 72 h after infection). The expression of amine oxidase<em> </em>(<em>AMO</em>)<em> </em>gene was observed highest in stem rot susceptible genotype TG37A while minimum in GJG31. Expression of this gene was remarkably induced in TG37A which may leads to higher accumulation of H<sub>2</sub>O<sub>2</sub>. Higher content of a polyamine, putrescine was found in the leaves of stem rot tolerant genotypes at 48 and 72 h after infection. These results implied that tolerant genotypes induced higher polyamine biosynthesis which may involve in plant defense and impart tolerance/ resistance. While, susceptible genotype (TG37A), utilized higher flux of S-Adenosyl methionine (SAM) for ethylene biosynthesis which may leads to necrosis of plants. Thus, stem rot resistant genotypes may be developed through genetic manipulation of polyamine biosynthesis pathway.</p>
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2021-08-02 15:25:09
 
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http://op.niscair.res.in/index.php/IJEB/article/view/53158
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 59, ##issue.no## 7 (2021): IJEB [JULY 2021]
 
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