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Novel application of Nerium leaf and Image J software in drop collapse assay for rapid screening of biosurfactant producing microorganisms

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Title Novel application of Nerium leaf and Image J software in drop collapse assay for rapid screening of biosurfactant producing microorganisms
 
Creator Mishra, Neelam
Agsar, Dayanand
Deepthi, Seelam Siva
Kattegouda, Umesh Madire
Shetty, Prakasham Reddy
Pandey, Vijyendra
 
Subject Cutin layer
Contact angle
Drop diameter
Critical micellar concentration
 
Description 484-492
Biosurfactants are attractive molecules with varied applicationsmainly oil degradation, emulsification, bioremediation,
therapeutics and conjugation of nanoparticles. The existing screening methods for biosurfactants are inappropriate and too
tedious. Here, we have explored a novel approach with drop collapse assay wherein we replaced the microtiter well plate
with the naturally hydrophobic Nerium (Nerium oleander L.) leaf. The stability of beaded drops on the leaf indicates
negative phenomenon, and spreading of drop indicates positive phenomenon for surfactant property, as confirmed by the
measuring drop diameter using Image J software. Fifty five bacterial cultures isolated from oil contaminated site were
screened through this novel approach which revealed that the isolates DNM49 (6.75±0.29 mm), DNM50 (7.45±0.19 mm)
and DNM51 (6.14±0.82 mm) were the best in terms of surface tension reduction, although thirty other isolates were also
found to be positive. A gradation of activity in terms of surface tension reduction was also established based on drop
diameter. The results demonstrated promising application of Nerium leaf with Image J software in drop collapse assay as an
eco-friendly and cost-effective and technically authenticated alternative to the existing assays.
 
Date 2021-08-05T08:55:20Z
2021-08-05T08:55:20Z
2021-07
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscair.res.in/handle/123456789/57850
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.59(07) [July 2021]