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Evaluation of free radical scavenging and anti-lipoxygenase activity in various fractions of ayurvedic polyherbal decoction, Punarnavadi kashayam

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Title Evaluation of free radical scavenging and anti-lipoxygenase activity in various fractions of ayurvedic polyherbal decoction, Punarnavadi kashayam
 
Creator M B, Bashi
S J, Rahitha Devi
B, Prakash Kumar
 
Subject Anti-inflammatory activity
Free radical scavenging activity
Lipoxygenases
Phenolic content
Phytochemicals
 
Description 651-659
Punarnavadi kashayam (PK), a classical ayurvedic polyherbal decoction prescribed for abating various inflammatory
conditions, was analysed for its total phytochemical content, free radical scavenging, and anti-inflammatory activity in order to
validate its traditional usage. PK was sequentially fractionated using solvents of different polarity and the preliminary
phytochemical screenings were performed. Free radical scavenging activity was determined using various biochemical assays while
the anti-inflammatory potential was evaluated by determining the anti-lipoxygenase activity using FOX assay. Ethyl acetate
fraction showed better free radical scavenging activity as compared to the corresponding positive controls. The same fraction
exhibited the better IC50 for 5- and 12-lipoxygenase inhibition assay than the positive control, showing its anti-inflammatory
property. The phytochemical analysis revealed a higher content of phytochemicals in ethyl acetate, dichloromethane and methanol
fractions which displayed the correlation between phenolic content and the bioactivity. Overall, PK can effectively scavenge free
radicals and inhibit lipoxygenase enzymes, thereby decreasing the production of free radicals and pro-inflammatory leukotrienes.
 
Date 2021-08-06T09:04:17Z
2021-08-06T09:04:17Z
2021-07
 
Type Article
 
Identifier 0975-1068 (Online); 0972-5938 (Print)
http://nopr.niscair.res.in/handle/123456789/57888
 
Language en
 
Relation Int Cl.21: A61K 31/05, A61K 31/44, A61K 38/00, A61K 45/06, A61P 29/02
 
Publisher NIScPR-CSIR, India
 
Source IJTK Vol.20(3) [July 2021]