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Identifying Anti-Oxidant Biosynthesis Genes in Pearl Millet [Pennisetum glaucum (L.) R. Br.] Using Genome—Wide Association Analysis

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Relation http://oar.icrisat.org/11869/
https://doi.org/10.3389/fpls.2021.599649
doi:10.3389/fpls.2021.599649
 
Title Identifying Anti-Oxidant Biosynthesis Genes in Pearl Millet [Pennisetum glaucum (L.) R. Br.] Using Genome—Wide Association Analysis
 
Creator Yadav, C B
Tokas, J
Yadav, D
Winters, A
Singh, R B
Yadav, R
Gangashetty, P I
Srivastava, R K
Yadav, R S
 
Subject Pearl Millet
Genetics and Genomics
Germplasm
 
Description Pearl millet [Pennisetum glaucum (L.) R Br.] is an important staple food crop in the
semi-arid tropics of Asia and Africa. It is a cereal grain that has the prospect to be
used as a substitute for wheat flour for celiac patients. It is an important antioxidant
food resource present with a wide range of phenolic compounds that are good sources
of natural antioxidants. The present study aimed to identify the total antioxidant content
of pearl millet flour and apply it to evaluate the antioxidant activity of its 222 genotypes
drawn randomly from the pearl millet inbred germplasm association panel (PMiGAP), a
world diversity panel of this crop. The total phenolic content (TPC) significantly correlated
with DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity (% inhibition),
which ranged from 2.32 to 112.45% and ferric-reducing antioxidant power (FRAP)
activity ranging from 21.68 to 179.66 (mg ascorbic acid eq./100 g). Genome-wide
association studies (GWAS) were conducted using 222 diverse accessions and 67 K
SNPs distributed across all the seven pearl millet chromosomes. Approximately, 218
SNPs were found to be strongly associated with DPPH and FRAP activity at high
confidence [–log (p) > 3.0–7.4]. Furthermore, flanking regions of significantly associated
SNPs were explored for candidate gene harvesting. This identified 18 candidate
genes related to antioxidant pathway genes (flavanone 7-O-beta-glycosyltransferase,
GDSL esterase/lipase, glutathione S-transferase) residing within or near the association
signal that can be selected for further functional characterization. Patterns of genetic
variability and the associated genes reported in this study are useful findings, which
would need further validation before their utilization in molecular breeding for high
antioxidant-containing pearl millet cultivars.
 
Publisher Frontiers Media
 
Date 2021-05
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11869/1/fpls-12-599649.pdf
Yadav, C B and Tokas, J and Yadav, D and Winters, A and Singh, R B and Yadav, R and Gangashetty, P I and Srivastava, R K and Yadav, R S (2021) Identifying Anti-Oxidant Biosynthesis Genes in Pearl Millet [Pennisetum glaucum (L.) R. Br.] Using Genome—Wide Association Analysis. Frontiers in Plant Science (TSI), 12. pp. 1-13. ISSN 1664-462X