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<p>Rapid and sensitive High-Performance Thin-Layer Chromatographic (HPTLC) method for identification and quantification of luteolin by densitometry in Kasamarda (<em>Cassia occidentalis </em>L.)</p>

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Title Statement <p>Rapid and sensitive High-Performance Thin-Layer Chromatographic (HPTLC) method for identification and quantification of luteolin by densitometry in Kasamarda (<em>Cassia occidentalis </em>L.)</p>
 
Added Entry - Uncontrolled Name GIRME, ABOLI ; Pharmanza Herbal Pvt. Ltd.
GHULE, CHETANA ; Pharmanza Herbal Pvt. Ltd.
GAIKAR, NILESH ; Pharmanza Herbal Pvt. Ltd.
HINGORANI, LAL ; Pharmanza Herbal Pvt. Ltd.
 
Uncontrolled Index Term Densitometry, HPTLC, Luteolin, Quantification
 
Summary, etc. <p>Kasamarda (<em>C. occidentalis</em> L.) is a traditional herb recently recognized as a potential nutraceutical in bone health. The current botanical nutraceutical regulations require consistent standardization for biological applications. The present study reported the standardization of bioactive flavonoid luteolin from <em>Cassia occidentalis </em>L. using validated high-performance thin-layer chromatographic (HPTLC) densitometric (DS) method. The mobile phase composition of toluene, ethyl acetate, and formic acid was optimized to separate and identify luteolin using silica gel 60F<sub>254</sub> aluminum plates. The densitometric (DS) scanning was performed at 353 nm. This HPTLC-DS method was further validated as per ICH guidelines. The linearity was 200–700 ng/band with a correlation coefficient value of 0.994. The LOD and LOQ were found to be 54.06 ng/band and 163.84 ng/band, respectively. The recovery (88.38% and 100.72%) and precision (RSD,&lt;5%) indicated method performance is robust and accurate for the routine analysis. Further, this bioactive flavonoid presence was confirmed and quantified by UV-spectrumin the sample matrix using this validated HPTLC-DS method. This HPTLC-DS method was robust, precise and accurate for quality control of active constituents present in <em>C. occidentalis</em> L.</p>
 
Publication, Distribution, Etc. Indian Journal of Traditional Knowledge (IJTK)
2021-08-18 15:09:53
 
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http://op.niscair.res.in/index.php/IJTK/article/view/32565
 
Data Source Entry Indian Journal of Traditional Knowledge (IJTK); ##issue.vol## 20, ##issue.no## 3 (2021): Indian Journal of Traditional Knowledge
 
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Nonspecific Relationship Entry http://op.niscair.res.in/index.php/IJTK/article/download/32565/465500716
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