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Isolation of Halophilic Bacteria and Their Screening for Extracellular Enzyme Production

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Title Isolation of Halophilic Bacteria and Their Screening for Extracellular Enzyme Production
 
Creator Yadav, Deepa
Singh, Anuradha
Mathur, Nupur
Agarwal, Aditi
Sharma, Jyoti
 
Subject Extracellular-enzymes
Extremophiles
Halophiles
Halophilic-enzymes
 
Description 617-622
Halophiles are those microorganisms which are found at high salt concentration. These microorganisms have the capability to form a wide array of bioactive substances that have different applications in various industries. This work focussed at the isolation and screening of various halophilic bacterial strains from different places in Rajasthan and Tamil Nadu. Using a complete medium with different salt concentrations, 70 halophilic bacteria were isolated from 4 different sites. These isolated bacteria were then examined and screened out for the synthesis of different extracellular enzymes, such as cellulase, lipase and amylase. Out of the total, 24 isolates were found positive for amylase, 9 for lipase and 16 for cellulase. These enzymes can perform the hydrolytic activity at high salt concentrations. The various halophilic bacterial strains isolated from various places show a prospective for use in a range of biotechnological and molecular biology experiments and the extremozymes obtained from these bacteria have great industrial importance.
 
Date 2021-09-01T08:54:54Z
2021-09-01T08:54:54Z
2021-08
 
Type Article
 
Identifier 0975-1084 (Online); 0022-4456 (Print)
http://nopr.niscair.res.in/handle/123456789/57971
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source JSIR Vol.80(07) [July 2021]