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Analytical validation of a modified turbidimetric assay to screen sulphur oxidizing bacteria

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Field Value
 
Relation https://eprints.cmfri.org.in/14717/
https://www.sciencedirect.com/science/article/pii/S016770122030405X
https://doi.org/10.1016/j.mimet.2020.105998
 
Title Analytical validation of a modified turbidimetric assay to screen sulphur
oxidizing bacteria
 
Creator Amala, P V
Sumithra, T G
Reshma, K J
Anju, F
Subramannian, Shinoj
Vijayagopal, P
 
Subject Bacteria
Microbiology
Fish Biotechnology
 
Description Conventional turbidimetric assay for sulphate determination was modified to 100 times lesser reaction volume
on a convenient format using microtitre plate based platform, targeting routine microbiological applications to
screen sulphur oxidizing bacteria (SOB) cultures. The modified assay was linear up to 1500 mg/L of sulphate
concentration, which is about 37.5 times more than that of conventional assay. Upon regression analysis, linear
equation y = 1.243× + 0.011 was obtained having R2 value of 0.998. The modified assay was fully validated in
terms of precision, limit of detection (LOD), limit of quantification (LOQ), sensitivity, selectivity and robustness
to assure the reliability during final applications. LOD and LOQ were found as 7.4 mg/L and 24.8 mg/L of
sulphate concentration respectively. Further, accuracy of the assay over routine SOB screening media components
was tested, and proved as reliable and suitable for the intended application.
 
Date 2020
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier https://eprints.cmfri.org.in/14717/1/Journal%20of%20Microbiological%20Methods_2020_Sumithra%20T%20G.pdf
Amala, P V and Sumithra, T G and Reshma, K J and Anju, F and Subramannian, Shinoj and Vijayagopal, P (2020) Analytical validation of a modified turbidimetric assay to screen sulphur oxidizing bacteria. Journal of Microbiological Method, 176. pp. 1-6.