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Genome‐wide miRNAs profiles of pearl millet under contrasting high vapor pressure deficit reveal their functional roles in drought stress adaptations

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Relation http://oar.icrisat.org/11911/
https://doi.org/10.1111/ppl.13521
doi:10.1111/ppl.13521
 
Title Genome‐wide miRNAs profiles of pearl millet under contrasting high vapor pressure deficit reveal their functional roles in drought stress adaptations
 
Creator Palakolanu, S R
Gupta, S
Yeshvekar, R K
Chakravartty, N
Kaliamoorthy, S
Shankhapal, A R
Vempati, A S
Kuriakose, B
Lekkala, S P
Philip, M
Perumal, R C
Lachagari, V B R
Bhatnagar‐Mathur, P
 
Subject Drought Tolerance
Pearl Millet
 
Description Pearl millet (Pennisetum glaucum [L.] R. Br.) is an important crop capable of growing in
harsh and marginal environments, with the highest degree of tolerance to drought
and heat stresses among cereals. Diverse germplasm of pearl millet shows a significant
phenotypic variation in response to abiotic stresses, making it a unique model to
study the mechanisms responsible for stress mitigation. The present study focuses
on identifying the physiological response of two pearl millet high-resolution cross
(HRC) genotypes, ICMR 1122 and ICMR 1152, in response to low and high vapor
pressure deficit (VPD). Under high VPD conditions, ICMR 1152 exhibited a lower
transpiration rate (Tr), higher transpiration efficiency, and lower root sap exudation
than ICMR 1122. Further, Pg-miRNAs expressed in the contrasting genotypes under
low and high VPD conditions were identified by deep sequencing analysis. A total of
116 known and 61 novel Pg-miRNAs were identified from ICMR 1152, while
26 known and six novel Pg-miRNAs were identified from ICMR 1122 genotypes,
respectively. While Pg-miR165, 168, 170, and 319 families exhibited significant differential
expression under low and high VPD conditions in both genotypes, ICMR
1152 showed abundant expression of Pg-miR167, Pg-miR172, Pg-miR396 PgmiR399,
Pg-miR862, Pg-miR868, Pg-miR950, Pg-miR5054, and Pg-miR7527 indicating
their direct and indirect role in root physiology and abiotic stress responses.
Drought responsive Pg-miRNA targets showed upregulation in response to high VPD
stress, further narrowing down the miRNAs involved in regulation of drought tolerance
in pearl millet.
 
Publisher Wiley
 
Date 2021-08
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11911/1/PgLEA.pdf
Palakolanu, S R and Gupta, S and Yeshvekar, R K and Chakravartty, N and Kaliamoorthy, S and Shankhapal, A R and Vempati, A S and Kuriakose, B and Lekkala, S P and Philip, M and Perumal, R C and Lachagari, V B R and Bhatnagar‐Mathur, P (2021) Genome‐wide miRNAs profiles of pearl millet under contrasting high vapor pressure deficit reveal their functional roles in drought stress adaptations. Physiologia Plantarum (TSI). pp. 1-17. ISSN 0031-9317