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Functional characterization of the promoter of pearl millet heat shock protein 10 (PgHsp10) in response to abiotic stresses in transgenic tobacco plants

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Relation http://oar.icrisat.org/11912/
https://doi.org/10.1016/j.ijbiomac.2020.04.069
doi:10.1016/j.ijbiomac.2020.04.069
 
Title Functional characterization of the promoter of pearl millet heat shock protein 10 (PgHsp10) in response to abiotic stresses in transgenic tobacco plants
 
Creator Kummari, D
Bhatnagar-Mathur, P
Sharma, K K
Vadez, V
Palakolanu, S R
 
Subject Abiotic Stress
Pearl Millet
 
Description In the present study, the promoter region of the pearlmillet heat shock protein 10 (PgHsp10) genewas cloned and
characterized. The PgHsp10 promoter (PgHsp10pro) sequence region has all the cis-motifs required for tissue and
abiotic stress inducibility. The complete PgHsp10pro (PgHsp10PC) region and a series of 5′ truncations of PgHsp10
(PgHsp10D1 and PgHsp10D2) and an antisense formof PgHsp10pro (PgHsp10AS)were cloned into a plant expression
vector (pMDC164) through gateway cloning. All four constructs were separately transformed into tobacco
through Agrobacterium-mediated genetic transformation, and PCR-confirmed transgenic plants progressed to
T1 and T2 generations. The T2 transgenic tobacco plants comprising all PgHsp10pro fragments were used for
GUS histochemical and qRT-PCR assays in different tissues under control and abiotic stresses. The PgHsp10PC
pro expression was specific to stemand seedlings under control conditions. Under different abiotic stresses, particularly
heat stress, PgHsp10PCpro had relatively higher activity than PgHsp10D1pro, PgHsp10D2pro and
PgHsp10ASpro. PgHsp10pro from a stress resilient crop like pearl millet responds positively to a range of abiotic
stresses, in particular heat, when expressed in heterologous plant systems such as tobacco. Hence, PgHsp10pro
appears to be a potential promoter candidate for developing heat and drought stress-tolerant crop plants.
 
Publisher Elsevier
 
Date 2020-04
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11912/1/39.%20Functional%20characterization.pdf
Kummari, D and Bhatnagar-Mathur, P and Sharma, K K and Vadez, V and Palakolanu, S R (2020) Functional characterization of the promoter of pearl millet heat shock protein 10 (PgHsp10) in response to abiotic stresses in transgenic tobacco plants. International Journal of Biological Macromolecules (TSI), 156. pp. 103-110. ISSN 0141-8130