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Overexpression of RNA ‐binding bacterial chaperones in rice leads to stay‐green phenotype, improved yield and tolerance to salt and drought stresses

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Relation http://oar.icrisat.org/11913/
https://doi.org/10.1111/ppl.13369
doi:10.1111/ppl.13369
 
Title Overexpression of RNA ‐binding bacterial chaperones in rice leads to stay‐green phenotype, improved yield and tolerance to salt and drought stresses
 
Creator Guddimalli, R
Somanaboina, A K
Palle, S R
Edupuganti, S
Kummari, D
Palakolanu, S R
Naravula, J
Gandra, J
Qureshi, I A
Marka, N
Polavarapu, R
Kishor, P B
 
Subject Drought Tolerance
 
Description Genes encoding bacterial cold shock proteins A (CspA, 213 bp) and B (CspB, 216 bp)
were isolated from Escherichia coli strain K12, which showed 100% homology with
gene sequences isolated from other bacterial species. In silico domain, analysis
showed eukaryotic conserved cold shock domain (CSD) and ribonuclease-binding
domain (RBD) indicating that they bind to RNA and are involved in temperature
stress tolerance. Overexpression of these two genes in E. coli resulted in higher
growth in presence of 200 mM NaCl and 300 mM mannitol. Western blot confirmed
the translational products of the two genes. Seedlings of indica rice were transformed
with Agrobacterium tumefaciens containing pCAMBIA1301 CspA and CspB
genes. Transgene integration was confirmed by β-glucuronidase (GUS) histochemical
assay, polymerase chain reaction (PCR) amplification, and gene copy number by
Southern blotting. Chlorophyll, proline, Na+, and K+ contents were higher in transgenics
exposed to 150 mM NaCl and drought (imposed by withholding water)
stresses during floral initiation stage. Catalase (CAT), superoxide dismutase (SOD),
and guaiacol peroxidase (GPX) activities increased, while malondialdehyde (MDA)
content was low in transgenics. Transgenics displayed increased root, shoot, and panicle
lengths, root dry mass, and a distinct stay-green (SGR) phenotype. Higher transcript
levels of CspA, CspB, SGR, chlorophyllase, isopentenyl adenine transferase
1 (IPT1), 9-cis-epoxycarotenoid dioxygenase (NCED), SOD, and sirtuin 1 (SIRT1) genes
were observed in transgenics compared to wild type plants (WT) under multiple
stresses. Present work indicates that bacterial chaperone proteins are capable of
imparting SGR phenotype, salt and drought stress tolerance alongside grain
improvement.
 
Publisher Wiley
 
Date 2021-02
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11913/1/ppl.13369%20%282%29.pdf
Guddimalli, R and Somanaboina, A K and Palle, S R and Edupuganti, S and Kummari, D and Palakolanu, S R and Naravula, J and Gandra, J and Qureshi, I A and Marka, N and Polavarapu, R and Kishor, P B (2021) Overexpression of RNA ‐binding bacterial chaperones in rice leads to stay‐green phenotype, improved yield and tolerance to salt and drought stresses. Physiologia Plantarum (TSI). pp. 1-18. ISSN 0031-9317