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<p>Bioactivity guided fractionation of <em>Icacina trichantha</em> Oliv. (Icacinaceae) tuber for antimalarial activity against <em>Plasmodium berghei</em> infected mice and GC-MS profile of bioactive fraction</p>

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Title Statement <p>Bioactivity guided fractionation of <em>Icacina trichantha</em> Oliv. (Icacinaceae) tuber for antimalarial activity against <em>Plasmodium berghei</em> infected mice and GC-MS profile of bioactive fraction</p>
 
Added Entry - Uncontrolled Name Adaka, Ifeyinwa Chioma; University of Nigeria, Nsukka
Uzor, Philip F.; University of Nigeria, Nsukka, 410001 Enugu State, Nigeria
Nwodo, Ngozi Justina; University of Nigeria, Nsukka,410001 Enugu State, Nigeria
 
Uncontrolled Index Term Antimalarial activity, Ethnomedicinal uses, GC-MS analysis, Icacina trichantha, Plasmodium berghei
 
Summary, etc. Aqueous methanol extract of the tuber of Icacina trichantha was prepared using cold maceration and dried in vacuo at 40°C. The extract was purified using solvent-solvent partitioning with n-hexane, dichloromethane, ethyl acetate, n-butanol. n-Hexane fraction was purified using Vacuum Liquid Chromatography, eluting with a gradient of dichloromethane in methanol (9:1, 7:3, 5:5, 0:10, each 500 mL) to obtain four sub-fractions. Acute toxicity study was done using Lorke’s method while in vivo anti-malarial study was carried out using suppressive model. Phytochemical analysis was carried out using standard procedure and most active sub-fraction was subjected to gas chromatography-mass spectroscopy. The extract at doses of 100, 200 and 400 mg/kg caused a significant (p&lt;0.001) increase in percentage suppression of Plasmodium: 91.54 %, 94.48 % and 94.58% respectively. Phytochemical analysis of the extract revealed the presence of alkaloids, tannins, flavonoids saponin, glycoside, terpenoids, phenols, steroids, carbohydrates, reducing sugars. The GC-MS analysis showed the presence of eighteen compounds, the most abundant compound includes 9- octadecenoic acid (Z)-, methyl ester (oleic acid, 15.30%), 9, 12-octadecadienoic acid (Z, Z), methyl ester (linoleic acid, 14.34%). These findings suggest scientific evidence in support to the use of I. trichantha tuber for the management of malaria.
 
Publication, Distribution, Etc. Indian Journal of Traditional Knowledge (IJTK)
2021-11-04 15:53:28
 
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http://op.niscair.res.in/index.php/IJTK/article/view/27188
 
Data Source Entry Indian Journal of Traditional Knowledge (IJTK); ##issue.vol## 20, ##issue.no## 4 (2021): Indian Journal of Traditional Knowledge
 
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Nonspecific Relationship Entry http://op.niscair.res.in/index.php/IJTK/article/download/27188/465484101