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Functional characterization of late embryogenesis abundant genes and promoters in pearl millet ( Pennisetum glaucum L.) for abiotic stress tolerance

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Relation http://oar.icrisat.org/11914/
https://doi.org/10.1111/ppl.13544
doi:10.1111/ppl.13544
 
Title Functional characterization of late embryogenesis abundant genes and promoters in pearl millet ( Pennisetum glaucum L.) for abiotic stress tolerance
 
Creator Divya, K
Palakolanu, S R
Kavi Kishor, P
Rajesh, A S
Vadez, V
Sharma, K K
Mathur, P B
 
Subject Abiotic Stress
Pearl Millet
 
Description Late embryogenesis abundant (LEA) genes display distinct functions in response to
abiotic stresses in plants. In pearl millet (Pennisetum glaucum L.), a total of 21 PgLEA
genes were identified and classified into six groups including LEA1, LEA2, LEA3, LEA5,
LEA7, and dehydrins (DHN). Open reading frames (ORFs) of PgLEAs range from
291 bp (PgLEA1-1) to 945 bp (PgLEA2-11) and distributed randomly among the seven
chromosomes. Phylogenetic analysis revealed that all PgLEA proteins are closely
related to sorghum LEA proteins. The PgLEAs were found to be expressed differentially
under high progressive vapor pressure deficit (VPD), PgLEA7 was significantly
expressed under high VPD and was selected for functional validation. In silico analysis
of the PgLEA promoter regions revealed abiotic stress-specific cis-acting elements
such as ABRE, CCAAT, MYBS, and LTRE. Based on the type of motifs, PgLEAPC promoter
(758 bp), its deletion 1 (PgLpd1, 349 bp) and deletion 2 (PgLpd2, 125 bp) were
cloned into the plant expression vector pMDC164 having the promoter-less uidA
gene. All the three plant expression vectors were introduced into tobacco through
Agrobacterium tumefaciens-mediated transformation to obtain T1 and T2 generations
of transgenic plants. Based on expression of the uidA gene, tissue-specific expression
was observed in mature stems, roots and seedlings of PgLEAPC and PgLpd1 carrying
transgenics only. While the transgenic PgLEAPC plants displayed significantly higher
uidA expression in the stem and root tissues under salt, drought, heat, and cold
stresses, very low or no expression was observed in PgLpd1 and PgLpd2 transgenics
under the tested stress conditions. The results of this study indicate that the complete
promoter of PgLEAPC plays a role in developing abiotic stress tolerance in
plants.
 
Publisher Wiley
 
Date 2021-08
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11914/1/44.%20Functional%20characterization.pdf
Divya, K and Palakolanu, S R and Kavi Kishor, P and Rajesh, A S and Vadez, V and Sharma, K K and Mathur, P B (2021) Functional characterization of late embryogenesis abundant genes and promoters in pearl millet ( Pennisetum glaucum L.) for abiotic stress tolerance. Physiologia Plantarum (TSI). pp. 1-13. ISSN 0031-9317