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Bioactivity guided isolation and characterization of antimicrobial principle from Hydrocotyle javanica Thunb.

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Title Bioactivity guided isolation and characterization of antimicrobial principle from Hydrocotyle javanica Thunb.
 
Creator Sood, Kaushal
Yadav, R N S
 
Subject Antimicrobial activity
Bioautography
Cytotoxicity
Hydrocotyle javanica
Structure
 
Description 913-919
Hydrocotyle javanica Thunb. (Family Araliaceae) has been used in traditional herbal medicine as a substitute of Centella asiatica (L.) Urban. Although, the latter has been studied extensively and various biologically active constituents have been reported, the former has not been studied for its active constituents. The current study followed activity guided isolation and identification of the fraction with antimicrobial activity from H. javanica. Plant extracts were prepared by extracting successively with petroleum ether, ethyl acetate and ethanol. Antimicrobial activity was evaluated using gram positive and gram negative bacteria and Candida albicans. Bioactivity-guided isolation was carried out by preparative thin layer chromatography and bioautography. Cytotoxicity of the most active antimicrobial principle was evaluated against Human embryonic kidney (HEK293) and Adenocarcinomic Human Alveolar Basal Epithelial (A549) cell lines. The tentative structure of the most active antimicrobial principle was constructed from the data obtained from FTIR, MS, H-NMR and C-NMR.
 
Date 2021-11-11T10:43:15Z
2021-11-11T10:43:15Z
2021-10
 
Type Article
 
Identifier 0975-1068 (Online); 0972-5938 (Print)
http://nopr.niscair.res.in/handle/123456789/58458
 
Language en
 
Relation Int Cl.21: A61K 36/25, A61K 45/06
 
Publisher CSIR-NIScPR, India
 
Source IJTK Vol.20(4) [October 2021]