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Co-Inoculation of Bacillus spp. for Growth Promotion and Iron Fortification in Sorghum

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Relation http://oar.icrisat.org/11915/
https://doi.org/10.3390/su132112091
doi:10.3390/su132112091
 
Title Co-Inoculation of Bacillus spp. for Growth Promotion and Iron Fortification in Sorghum
 
Creator Manasa, M
Ravinder, P
Gopalakrishnan, S
Srinivas, V
Sayyed, R Z
El Enshasy, H A
Yahayu, M
Kee Zuan, A T
Kassem, H S
Hameeda, B
 
Subject Biofortification
Sorghum
 
Description Seven Bacillus spp. isolated from the marine water and the rhizosphere of the medicinal
plant Coscinium fenestratum were studied to produce plant growth promotion (PGP) traits in vitro.
Among the seven isolates, MMRH22 and RHPR20 produced copious amounts of PGP traits. Based
on the 16S rRNA sequence, the two potent bacterial isolates, RHPR20 and MMRH22, were identified
as Bacillus mojavensis and Bacillus cereus, respectively. A compatibility test between the isolates
RHPR20 and MMRH22 revealed they are compatible and can be used as a consortium. Both isolates
were evaluated for the plant growth promotion and the biofortification of sorghum under greenhouse
conditions. Treatments included the application of MMRH22, RHPR20, their consortium
(RHPR20 + MMRH22), and an uninoculated control. Inoculation with bacterial cultures resulted in
a significant increase in the plant height; the number of leaves; the leaf area; the root, shoot, and
leaf weight; and the yield of sorghum at 30 and 60 days after sowing (DAS). The scanning electron
micrograph of the sorghum plant roots revealed extensive colonization in the plants treated with the
bacterial cultures compared to the uninoculated control. The sorghum grains obtained after final
harvest were analyzed for their nutrient content by ICP–OES. The biofortification in sorghum grains
was varied and was found to enhance the iron content up to 97%. This study revealed that treatments
with microbial consortia enhance plant growth, yield, and iron content, which could combat nutrient
deficiencies in plants and humans.
 
Publisher MDPI
 
Date 2021-11
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11915/1/Manasa%20et%20al%202021%20Published%20article.pdf
Manasa, M and Ravinder, P and Gopalakrishnan, S and Srinivas, V and Sayyed, R Z and El Enshasy, H A and Yahayu, M and Kee Zuan, A T and Kassem, H S and Hameeda, B (2021) Co-Inoculation of Bacillus spp. for Growth Promotion and Iron Fortification in Sorghum. Sustainability (TSI), 13 (21). pp. 1-14. ISSN 2071-1050