Record Details

Characterization of rhizobia isolated from leguminous plants and their impact on the growth of ICCV 2 variety of chickpea (Cicer arietinum L.)

OAR@ICRISAT

View Archive Info
 
 
Field Value
 
Relation http://oar.icrisat.org/11916/
https://doi.org/10.1016/j.heliyon.2021.e08321
doi:10.1016/j.heliyon.2021.e08321
 
Title Characterization of rhizobia isolated from leguminous plants and their impact on the growth of ICCV 2 variety of chickpea (Cicer arietinum L.)
 
Creator Mir, M I
Kumar, B K
Gopalakrishnan, S
Vadlamudi, S
Hameeda, B
 
Subject Chickpea
Legume Crops
 
Description Six rhizobia-like-bacterial strains in total, secluded from the root and stem nodules of various leguminous plants
were characterized for growth promoting ability on ICCV 2 variety of chickpea. Bacterial strains showed production
of IAA, NH3, siderophore, HCN, ACC deaminase, hydrolytic enzyme production such as chitinase,
amylase, protease, lipase, β-1, 3-glucanase and solubilization of nutrients such as phosphate, zinc and potassium.
However the performance of PGP traits characterized in-vitro varied among the six bacterial strains. The sequences
of 16S rRNA gene of bacterial strains IHSR, IHRG, IHAA, IHGN-3, IHCP-1 and IHCP-2 showed maximum identity
with Rhizobium sp., Rhizobium tropici, Rhizobium multihospitium, Mesorhizobium sp., Burkholderia cepacia and
Rhizobium pusense. In plate culture conditions the bacterial strains changed the colour of media (NFB) from green
to blue and showed amplification of nifH gene by PCR, and also enhanced nodule formation in chickpea under
greenhouse conditions, which explains their nitrogen fixing ability. Scanning electron microscopy studies of
chickpea roots showed colonization by all the six bacterial strains in solo and by consortium (IHRG þ IHGN-3).
Under greenhouse conditions, chickpea plants inoculated with different strains showed improvement in plant
height, number of branches, total chlorophyll, nodule number, nodule weight, shoot weight, root weight, root
volume and root surface area at 30 and 45 days after sowing (DAS) over the uninoculated control plants. It was
also observed at the crop maturity stage all the bacterial strains inoculated separately enhanced pod number, seed
number and total NPK compared to uninoculated control plants. This study suggests that bacteria associated with
root and stem nodules can be a promising resource to enhance nodulation, PGP and crop yields in chickpea.
 
Publisher Elsevier
 
Date 2021-11
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11916/1/PIIS2405844021024245%20%281%29.pdf
Mir, M I and Kumar, B K and Gopalakrishnan, S and Vadlamudi, S and Hameeda, B (2021) Characterization of rhizobia isolated from leguminous plants and their impact on the growth of ICCV 2 variety of chickpea (Cicer arietinum L.). Heliyon (TSI), 7 (11). pp. 1-13. ISSN 2405-8440