Record Details

Production and identification of intricate bioactive oligosaccharides from Nyctanthesarbor-tristis leaves by a combination of enzymatic, HPAEC and MALDI-TOF-MS techniques

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Production and identification of intricate bioactive oligosaccharides from Nyctanthesarbor-tristis leaves by a combination of enzymatic, HPAEC and MALDI-TOF-MS techniques
 
Creator Ali, Imran
Mukherjee, Shuvam
Jana, Subrata
Khawas, Sadhana
Ray, Bimalendu
Ray, Sayani
 
Subject N. arbor-tristis leaves
hemicelluloses
oligosaccharides
HPAEC
MALDI-TOF-MS
 
Description 1471-1477
Non-digestible oligosaccharides are associated with positive health outcomes; however, hemicellulosic oligosaccharides
of N. arbor-tristis have not been studied. Herein, we report production of hemicellulosic oligosaccharides from its leaves
and their identification. A series of complex neutral oligosaccharides (1-16), produced by endo-(1,4)--D-glucanase
digestion of the alkali extracted xyloglucan, have been identified by chemical, HPAE chromatographic and MALDI-mass
spectrometric techniques. Additionally, xylan enriched hemicellulose-A fraction upon endo-(1,4)--D-xylanase digestion
yield several acidic and neutral xylo-oligosaccharides (17-24), each containing a backbone of -(1,4)-D-Xylp units
substituted at position 2 with a single stub of D-Xylp or 4-O-Me-D-GlcpA residues. The structural diversity and high water
solubility of these enzyme-generated oligosaccharides suggested promising health implications.
 
Date 2021-11-16T09:30:47Z
2021-11-16T09:30:47Z
2021-11
 
Type Article
 
Identifier 0975-0983(Online); 0376-4699(Print)
http://nopr.niscair.res.in/handle/123456789/58512
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJC-B Vol.60B(11) [November 2021]