Record Details

Increased tolerance to photooxidative stress by overexpression of mitochondrial superoxide dismutase in transgenic tobacco

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Increased tolerance to photooxidative stress by overexpression of mitochondrial superoxide dismutase in transgenic tobacco
 
Creator Morita, Shigeto
Kato, Masato
Nakayama, Yousuke
Yokoyama, Hidefumi
Kaminaka, Hironori
Masumura, Takehiro
Tanaka, Kunisuke
Satoh, Shigeru
 
Subject Photooxidative stress
reactive oxygen
superoxide dismutase
stress tolerance
tobacco
 
Description 76-80
Under abiotic stress conditions, plants suffer from oxidative damage caused by increased generation of reactive oxygen species (ROS). Although chloroplasts are the main source of ROS generation in plant cells, changes in ROS level in mitochondria affect plant stress response. In this study, we investigated whether overexpression of mitochondrial manganese superoxide dismutase (Mn-SOD) can enhance tolerance to photooxidative stress. We produced transgenic tobacco plants overexpressing rice Mn-SOD under the control of cauliflower mosaic virus 35S promoter, and assessed the stress tolerance of those plants. The transgenic lines showed higher total SOD activity by 2.8 to 5.2 fold than wild type plants. Chlorophyll fluorescence measurement revealed that the stress-induced inhibition of photosystem II was alleviated under high light, chilling and heat stresses in the transgenic lines. This result indicates that the Mn-SOD contributes to the defense against photooxidative damages in chloroplasts under these stress conditions.
 
Date 2021-11-17T10:54:04Z
2021-11-17T10:54:04Z
2021-01
 
Type Article
 
Identifier 0975-0967 (Online); 0972-5849 (Print)
http://nopr.niscair.res.in/handle/123456789/58535
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBT Vol.20(1) [January 2021]