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A strategy to develop disabled infectious single-cycle (DISC) foot and mouth disease virus by 3B3 gene deletion using the infective cDNA copy of the genome

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Title A strategy to develop disabled infectious single-cycle (DISC) foot and mouth disease virus by 3B3 gene deletion using the infective cDNA copy of the genome
 
Creator Sarkar, Swaroop
Suryanarayana, V V S
Sekar, S Chandra
Shankar, S R Madan
Reddy, G R
Dechamma, H J
 
Subject Disabled infectious single cycle
3B3 gene deleted replicon
BHK cells expressing 3B3
FMDV virulence
attenuation.
 
Description 35-42
Disabled infectious single-cycle (DISC) virus is in between attenuated and inactivated. When used as a vaccine DISC
virus behaves like inactivated virus as it cannot further multiply in the vaccinated individual after one cycle of replication.
When infected to permissive cells expressing virus specific protein which has been deleted from viral genome, the virus
replicates normally. The development of DISC virus involves the deletion of an open reading frame (ORF) coding for a key
protein involved in the viral replication or viral capsid formation. Such virus, when injected in animals, can complete only
one round of replication without producing a progeny virus. Here we report similar strategy followed for the production
DISC foot and mouth disease virus (FMDV). We have selected 3B3 protein gene that expresses 3 copies of virus specific
genome-linked protein needed for virus replication and deleted from the FMDV replicon carrying FMDV serotype Asia 1
backbone and inserted the same into baby hamster kidney 21 (BHK-21) cell genome. Upon transfection of the genetically
modified BHK cells with RNA copy of the genetically modified cDNA, replication of the virus started in these cells. The
DISC virus so developed can be a potent vaccine candidate for achieving robust and long duration of immune response
against FMDV infection in bovine. The approach also took care in the development of serotype specific DISC viruses using
single FMDV Asia 1 replicon. Vaccines based on DISC viruses may be superior in terms of immune response as compared
to inactivated vaccines.
 
Date 2021-11-17T11:22:30Z
2021-11-17T11:22:30Z
2021-01
 
Type Article
 
Identifier 0975-0967 (Online); 0972-5849 (Print)
http://nopr.niscair.res.in/handle/123456789/58539
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBT Vol.20(1) [January 2021]