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<p>Production and identification of intricate bioactive oligosaccharides from <em>Nyctanthesarbor-tristis</em> leaves by a combination of enzymatic, HPAEC and MALDI-TOF-MS techniques</p><p> </p>

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Title Statement <p>Production and identification of intricate bioactive oligosaccharides from <em>Nyctanthesarbor-tristis</em> leaves by a combination of enzymatic, HPAEC and MALDI-TOF-MS techniques</p><p> </p>
 
Added Entry - Uncontrolled Name Ray, Sayani ; Natural Products Laboratory, Department of Chemistry, The University of Burdwan, Golapbag Campus, Purba Bardhaman 713 104, India
Ali, Imran ; Natural Products Laboratory, Department of Chemistry, The University of Burdwan, Golapbag Campus, Purba Bardhaman 713 104, India
Mukherjee, Shuvam ; Natural Products Laboratory, Department of Chemistry, The University of Burdwan, Golapbag Campus, Purba Bardhaman 713 104, India
Jana, Subrata ; Natural Products Laboratory, Department of Chemistry, The University of Burdwan, Golapbag Campus, Purba Bardhaman 713 104, India
Khawas, Sadhana ; Natural Products Laboratory, Department of Chemistry, The University of Burdwan, Golapbag Campus, Purba Bardhaman 713 104, India
Ray, Bimalendu ; Natural Products Laboratory, Department of Chemistry, The University of Burdwan, Golapbag Campus, Purba Bardhaman 713 104, India
 
Uncontrolled Index Term N. arbor-tristis leaves, hemicelluloses, oligosaccharides, HPAEC, MALDI-TOF-MS
 
Summary, etc. <p>Nondigestible oligosaccharides are associated with positive health outcomes; however, hemicellulosic oligosaccharides of <em>N. arbor-tristis</em> have not been studied. Herein we report production hemicellulosic oligosaccharides from its leaves and their identification. A series of complex neutral oligosaccharides (<strong>1-16</strong>), produced by <em>endo</em>-(1,4)-b-d-cellulase digestion of the alkali extracted xyloglucan, were identified by chemical, HPAE chromatographic and MALDI mass spectrometric techniques. Additionally, xylan enriched hemicellulose-A fraction upon <em>endo</em>-(1,4)-b-d-xylanase digestion yielded several acidic and neutral xylo-oligosaccharides (<strong>17-24</strong>), each containing a backbone of b-(1,4)-d-Xyl<em>p</em> units substituted at position 2 with a single stub of d-Xyl<em>p</em> and 4-<em>O</em>-Me-d-Glc<em>p</em>A residues. The structural diversity and high-water solubility of these enzyme-generated oligosaccharides implied promising health implication.</p>
 
Publication, Distribution, Etc. Indian Journal of Chemistry -Section B (IJC-B)
2021-11-18 10:12:23
 
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http://op.niscair.res.in/index.php/IJCB/article/view/33379
 
Data Source Entry Indian Journal of Chemistry -Section B (IJC-B); ##issue.vol## 60, ##issue.no## 11 (2021): Indian Journal of Chemistry- Section-B, (IJCB)
 
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