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<p>Applicability of Native L-Arginase produced by <em>Streptomyces plicatus</em> KAR73 as Antineoplastic Agent</p>

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Title Statement <p>Applicability of Native L-Arginase produced by <em>Streptomyces plicatus</em> KAR73 as Antineoplastic Agent</p>
 
Added Entry - Uncontrolled Name Gumashta, Raghvendra ; People’s College of Medical Sciences and Research Centre, People’s University, Bhopal. M.P., 462037
Jain, Richa ; Centre for Scientific Research and Development, People’s University, Bhopal. M.P., 462037
Pandey, Akanksha ; Centre for Scientific Research and Development, People’s University, Bhopal. M.P., 462037
Tiwari, Prachi ; Centre for Scientific Research and Development, People’s University, Bhopal. M.P., 462037
Jain, Aakanchha ; Bhagyoday Tirth Pharmacy College, Sagar; NIPER Ahmedabad
Madhya Pradesh Council of Science and Technology, Bhopal
 
Uncontrolled Index Term Anticancer, Apoptosis, Arginase, Cytotoxicity, Mammary tumor
 
Summary, etc. <p>Most of the cancer cells require high quantity of arginine for sustaining their fast-metabolic rates. Limiting supply of arginine to cancer cells using arginase may prove to be of great therapeutic value. The arginase produced by a micro organism isolated from soil has been used in industrial production of ornithine, however its use in anticancer activities is scarcely studied. This study optimized soybean meal supplemented basal media for Arginase production. Arginase was purified using ammonium sulphate precipitation, sephadex G-100 column chromatography and DEAE chromatography achieving 79.2% purification fold and 24.26% yield. It had 23 KD molecular weight as determined using Native PAGE and was active at considerably wide pH range of 6–10 and temperature 30–50℃. Whereas the maximum arginase activity was noticed with Mn²<sup>+ </sup>ions followed by polyvinyl pyrrolidine (PVP) at 70 mM substrate concentration, the maximum inhibition of activity was caused by CuC. <em>Streptomyces plicatus</em> KAR 73 produced arginase on mouse mammary cell line (CID 9) was not inhibited by the arginase upto 6.5 U/mL. Significant (p&lt; 0.001) inhibition in Mouse mammary tumor (C1271) cell lines was observed with IC<sub>50</sub> 5.2 U/mL. The ornithine has been produced earlier with <em>Mycoplasma </em>and <em>Clostridium</em> by other researchers but production of native arginase from <em>Streptomyces</em> specifically for anticancer activities has not yet been reported. The present study infers that Arginase produced from native <em>Streptomyces</em> has shown promising results thereby enabling feasibility assessment towards cost effective industrial production of arginase.</p>
 
Publication, Distribution, Etc. Journal of Scientific and Industrial Research (JSIR)
2021-11-29 17:02:53
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/JSIR/article/view/37319
 
Data Source Entry Journal of Scientific and Industrial Research (JSIR); ##issue.vol## 80, ##issue.no## 10 (2021): Journal of Scientific and Industrial Research
 
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Nonspecific Relationship Entry http://op.niscair.res.in/index.php/JSIR/article/download/37319/465515846
http://op.niscair.res.in/index.php/JSIR/article/download/37319/465515847