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Low temperature co-fired ceramic (LTCC)based biosensor for detection of vanadium using immobilized Arachis hypogaea alkaline phosphatase on multi walled carbon nanotubes ethyl cellulose sponge matrix

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Title Low temperature co-fired ceramic (LTCC)based biosensor for detection of vanadium using immobilized Arachis hypogaea alkaline phosphatase on multi walled carbon nanotubes ethyl cellulose sponge matrix
 
Creator Giramkar, Vijaya D.
Phatak, Girish J.
Sabharwal, Sushma G.
 
Subject Alkaline phosphatase
Electronic packaging
Heavy metal toxicity
MWCNTs
Thermometric sensor
 
Description 916-920
Studies on enzyme based thermometric biosensor are limited.
Here, we report on fabrication of an alkaline phosphate based
thermometric biosensor. We designed alkaline phosphatase
inhibition based biosensor for detection of vanadium using
immobilized alkaline phosphatase on multi walled carbon nano
tubes (MWCTs) ethyl cellulose sponge matrix. We isolated
protein from plant source, partially purified and fabricated a
miniature ceramic viz. LTCC (low temperature co-fired ceramics
technology) based biosensor for detection of vanadium. This
biosensor consists of a microreaction chamber with buried
heaters. Alkaline phosphatase has been isolated from the seeds of
‘Arachis hypoghaea’ was studied for its biochemical properties
viz. optimum pH and temperature. The partially purified enzyme
was immobilized using carboxyl-functionalised carbon nanotubes
(CNTs) by cross linking with epichlorohydrin (ECH) along with a
matrix of ethyl cellulose. The developed LTCC based biosensor
on testing indicated its linear response to vanadium concentration
up to 9 mM with a relatively high sensitivity of about
147 nA/mM. Thus, we have demonstrated a LTCC based
biosensor using immobilized alkaline phosphatase for detection of
vanadium.
 
Date 2021-12-02T06:07:02Z
2021-12-02T06:07:02Z
2021-12
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscair.res.in/handle/123456789/58563
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.59(12) [December 2021]