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Optimization of growth conditions for maximum hexavalent chromium reduction by the microbial consortium isolated from chromite mines

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Title Optimization of growth conditions for maximum hexavalent chromium reduction by the microbial consortium isolated from chromite mines
 
Creator J, Leonard
Mishra, Susmita
 
Subject Bioremediation
Box-Behnken design (BBD)
Heavy metal pollution
Response surface methodology (RSM)
Serratia nematodiphila
 
Description 867-876
Hexavalent chromium (Cr(VI)) contamination is one of the important threat to the environment. Detoxification of Cr(VI)
can be achieved with the use of chromium resistant bacteria. Three chromium resistant organisms were isolated from the soil
of chromite mines and identified as Serratia nematodiphila, Bacillus cereus and Bacillus sp. SDIP3 using 16S rRNA
sequencing. A consortium was developed with the isolated bacterial strains after the acclimatisation. The effect of
environmental factors, such as temperature, pH and nutrient sources were studied for the maximum chromium reduction by
the consortium at the Cr(VI) concentration of 25 µg/mL. The reduction of Cr(VI) was optimum at the temperature 35ºC and
pH 7. The Cr(VI) reduction was more effective with the glucose as carbon source, inoculum age of 24 h and 4% of inoculum
volume. The statistical optimisation of parameters such as pH, temperature and carbon source concentration for the
maximum reduction of Cr(VI) was done by Response surface methodology using Box-Behnken design (BBD). The
consortium has shown 92% reduction of Cr(VI) under the optimised conditions. This consortium was further used in
continuous reactor system to reduce the Cr(VI) to less toxic trivalent chromium.
 
Date 2021-12-02T06:32:53Z
2021-12-02T06:32:53Z
2021-12
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscair.res.in/handle/123456789/58569
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.59(12) [December 2021]